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Fluorescent Measurement of TNS Binding to Calmodulin: A Biochemistry Laboratory Experiment
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Abstract
This article describes a method for the determination of the sites of binding of a fluorescent probe to a protein. The protein examined is Calmodulin.
Keywords (Audience):
Second-Year UndergraduateKeywords (Domain):
BiochemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Proteins / PeptidesCiting Articles
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This article has been cited by 5 ACS Journal articles (5 most recent appear below).
Measuring Binding Affinity of Protein−Ligand Interaction Using Spectrophotometry: Binding of Neutral Red to Riboflavin-Binding Protein
Pirom Chenprakhon, Jeerus Sucharitakul, Bhinyo Panijpan, Pimchai ChaiyenJournal of Chemical Education2010 87 (8), 829-831Measuring Binding Affinity of Protein−Ligand Interaction Using Spectrophotometry: Binding of Neutral Red to Riboflavin-Binding Protein
Pirom Chenprakhon, Jeerus Sucharitakul, Bhinyo Panijpan, Pimchai ChaiyenJournal of Chemical Education2010 87 (8), 829-831The dissociation constant, Kd, of the binding of riboflavin-binding protein (RP) with neutral red (NR) can be determined by titrating RP to a fixed concentration of NR. Upon adding RP to the NR solution, the maximum absorption peak of NR shifts to 545 nm ...
Quantitative Determination of DNA–Ligand Binding Using Fluorescence Spectroscopy
Eamonn F. HealyJournal of Chemical Education2007 84 (8), 1304Quantitative Determination of DNA–Ligand Binding Using Fluorescence Spectroscopy
Eamonn F. HealyJournal of Chemical Education2007 84 (8), 1304DAPI, 4′,6-diamidino-2-phenylindole, binds to double-stranded DNA forming a complex that fluoresces up to twenty times more than DAPI alone. It is widely used in biochemical and cytochemical studies for DNA detection. The experiment described here ...
Fluorescence Measurement of Pyrene Wall Adsorption and Pyrene Association with Humic Acids. An Experiment for Physical Chemistry or Instrumental Methods
Edward C. Shane , Miranda Price-Everett and Tonya HansonJournal of Chemical Education2000 77 (12), 1617Fluorescence Measurement of Pyrene Wall Adsorption and Pyrene Association with Humic Acids. An Experiment for Physical Chemistry or Instrumental Methods
Edward C. Shane , Miranda Price-Everett and Tonya HansonJournal of Chemical Education2000 77 (12), 1617We describe a two-part fluorescence experiment that measures (i) the surface adsorption of the polycyclic aromatic hydrocarbon (PAH) pyrene to Pyrex, quartz, and LDPE surfaces and (ii) the binding of pyrene to natural humic acids (HA). A model for surface ...
A Visual Demonstration of Supramolecular Chemistry: Observable Fluorescence Enhancement upon Guest-Host Inclusion
Brian D. Wagner , Penny J. MacDonald and Maryam Wagner , Thomas A. BettsJournal of Chemical Education2000 77 (2), 178A Visual Demonstration of Supramolecular Chemistry: Observable Fluorescence Enhancement upon Guest-Host Inclusion
Brian D. Wagner , Penny J. MacDonald and Maryam Wagner , Thomas A. BettsJournal of Chemical Education2000 77 (2), 178Supramolecular chemistry, or "chemistry beyond the molecule", is an increasingly important area of modern chemical research. It deals with highly organized structures involving two or more individual molecules held together by intermolecular forces. ...
Resonance Energy Transfer in Peptides: A Biophysical Laboratory Experiment
Anne M. Hundzinski and Bruce D. AndersonJournal of Chemical Education1999 76 (3), 416Resonance Energy Transfer in Peptides: A Biophysical Laboratory Experiment
Anne M. Hundzinski and Bruce D. AndersonJournal of Chemical Education1999 76 (3), 416Fluorescence excitation and absorption spectra are used to calculate the efficiency of resonance energy transfer and the distance between two chromophores within a small peptide. In this investigation, tyrosine is the donor chromophore and tryptophan is ...
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- Received: August 03, 2009
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