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The Analysis of Riboflavin in Urine Using Fluorescence
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Abstract
To become functional as scientists, chemistry students must integrate concepts learned in their classes and apply them to novel, "real life" situations. The laboratory provides an important place for the students to practice integrating concepts. This laboratory experiment, designed for undergraduate biochemistry students, requires each student to determine the amount of riboflavin excreted by his/her body following oral administration of riboflavin contained in a multi-vitamin tablet. The experimental procedure describes a protocol for the analysis of riboflavin concentration in urine using a fluorometric assay. The students must draw upon their knowledge of solution preparation, construction of a standard curve, and back-calculation procedures to determine the concentration of riboflavin in their urine. Students need to combine knowledge from general and analytical chemistry with that learned in biochemistry to complete this analysis, thus providing an opportunity to integrate knowledge while answering a novel question.
Keywords (Audience):
Second-Year UndergraduateKeywords (Domain):
BiochemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Fluorescence SpectroscopyCiting Articles
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This article has been cited by 3 ACS Journal articles (3 most recent appear below).
Integrating Advanced High School Chemistry Research with Organic Chemistry and Instrumental Methods of Analysis
Brian J. KennedyJournal of Chemical Education2008 85 (3), 393Integrating Advanced High School Chemistry Research with Organic Chemistry and Instrumental Methods of Analysis
Brian J. KennedyJournal of Chemical Education2008 85 (3), 393This paper describes and discusses the unique chemistry course opportunities beyond the advanced placement-level available at a science and technology magnet high school. Students may select entry-level courses such as honors and advanced placement ...
An Inexpensive Device for Capillary Electrophoresis with Fluorescence Detection
Jonathan E. Thompson and Khriesto Shurrush , Greg AndersonJournal of Chemical Education2006 83 (11), 1677An Inexpensive Device for Capillary Electrophoresis with Fluorescence Detection
Jonathan E. Thompson and Khriesto Shurrush , Greg AndersonJournal of Chemical Education2006 83 (11), 1677We describe an inexpensive device for performing capillary electrophoresis (CE) separations with fluorescence detection. As a demonstration of the device's utility we have determined the mass of riboflavin in a commercially available dietary supplement. ...
Resonance Energy Transfer in Peptides: A Biophysical Laboratory Experiment
Anne M. Hundzinski and Bruce D. AndersonJournal of Chemical Education1999 76 (3), 416Resonance Energy Transfer in Peptides: A Biophysical Laboratory Experiment
Anne M. Hundzinski and Bruce D. AndersonJournal of Chemical Education1999 76 (3), 416Fluorescence excitation and absorption spectra are used to calculate the efficiency of resonance energy transfer and the distance between two chromophores within a small peptide. In this investigation, tyrosine is the donor chromophore and tryptophan is ...
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- Received: August 03, 2009
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