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A Simulated Growth Hormone Analysis

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Mary Harris

John Burroughs School, 755 South Price Road, St. Louis, MO 63124

J. Chem. Educ., 1996, 73 (8), p 735

DOI: 10.1021/ed073p735

Publication Date (Web): August 1, 1996

Section:

History, Education, and Documentation

Abstract

Growth hormone is a drug that is sometimes abused by amateur or professional athletes for performance-enhancement. This laboratory is a semimicroscale simulation analysis of a sample of "urine" to detect proteins of two very different molecular weights. Gel filtration uses a 10 mL disposable pipette packed with Sephadex. Students analyze the fractions from the filtration by comparing colors of the Brilliant Blue Coomassie Dye as it interacts with the proteins in the sample to a standard set of known concentration of protein with the dye. The simulated analysis of growth hormone is intended to be included in a unit on organic chemistry or in the second year of high school chemistry.

Keywords (Audience):

First-Year Undergraduate / General

Keywords (Domain):

Organic Chemistry

Keywords (Feature):

Filtrates and Residues

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Hormones

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

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- Measurement of Iron in Egg Yolk: An Instrumental Analysis Experiment Using Biochemical Principles
  Kevin M. Maloney , Emmanuel M. Quiazon and Ramee Indralingam
  Journal of Chemical Education2008 85 (3), 399
  The generally accepted method to determine iron content in food is by acid digestion or dry ashing and subsequent flame atomic absorption spectrometry or inductively coupled plasma atomic emission spectrometry. We have developed an experiment that ...
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