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Thin-Layer Chromatography Experiments That Illustrate General Problems in Chromatography

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M. Lederer and E. Leipzig-Pagani

Universite de Lausanne, Institut de Chimie Minerale et Analytique, Boite Postale 115, Centre Universitaire, CH-1015, Lausanne 15, Switzerland

J. Chem. Educ., 1996, 73 (10), p 974

DOI: 10.1021/ed073p974

Publication Date (Web): October 1, 1996

Abstract

A number of chromatography experiments using cellulose thin layers and aqueous eluents are proposed to illustrate:

1. How chromatography can be used for "pattern recognition" of the inks of felt-tip pens.

2. The principle of displacement chromatography of the constituents of the antiseptic Mercurochrome.

3. Salting-out chromatography for the separation of halides using a nearly saturated ammonium sulfate solution as eluent.

4. The impossibility to use chromatography alone as a method of identification: Methyl Orange travels differently in presence and absence of cyclodextrins or starches in the sample to be analysed.

Keywords (Audience):

First-Year Undergraduate / General

Keywords (Domain):

Laboratory Instruction

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Chromatography

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This article has been cited by 2 ACS Journal articles (2 most recent appear below).

Analysis and Identification of Acid–Base Indicator Dyes by Thin-Layer Chromatography
Daniel D. Clark
Journal of Chemical Education2007 84 (7), 1186
- Analysis and Identification of Acid–Base Indicator Dyes by Thin-Layer Chromatography
  Daniel D. Clark
  Journal of Chemical Education2007 84 (7), 1186
  Thin-layer chromatography (TLC) is a simple and useful technique for the separation of compounds in a mixture. Organic chemists commonly employ TLC to follow the progression of a reaction, to qualitatively analyze a compound or mixture, to determine ...
  Abstract | Hi-Res PDF | PDF w/ Links
The Preparation of a Fluorescent Dye: A Microscale TLC Experiment
Daniel S. Calimente , Shannon M. Strand , Shao-Chieh Chang and David E. Lewis
Journal of Chemical Education1999 76 (1), 82
- The Preparation of a Fluorescent Dye: A Microscale TLC Experiment
  Daniel S. Calimente , Shannon M. Strand , Shao-Chieh Chang and David E. Lewis
  Journal of Chemical Education1999 76 (1), 82
  This paper describes an experiment that allows the instructor to introduce TLC as a technique for analyzing reaction mixtures by monitoring the formation of a fluorescent dye. The experiment also illustrates the effects of eluting solvent on elution ...
  Abstract | Hi-Res PDF | PDF w/ Links