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Thin-Layer Chromatography: Four Simple Activities for Undergraduate Students
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Abstract
To simplify the conventional thin-layer chromatography, four TLC exercises are described which are easy to perform in a high school or college laboratory. In the first exercise, in place of a conventional TLC plate, an ordinary glass test tube, coated with the adsorbent, was used. In the second, the solvent was fed through a glass capillary, fused in the center of a horizontal TLC plate. Third exercise involved a burette to apply the solvent in the center of a TLC plate. In fourth exercise, which resolved the mixture most rapidly, the TLC plate was rotated with the help of an electric motor and solvent was applied by a burette in the center. In order to compare the results a single set of adsorbent, samples and mobile phase was used throughout the work. The described TLC exercises are generally more simple, inexpensive, faster and reproducible than conventional ascending TLC. The purpose of this work was to simplify conventional TLC exercises so that this important analytical technique could be introduced at the undergraduate level in relatively less developed countries.
Keywords (Audience):
High School / Introductory ChemistryKeywords (Domain):
Inorganic ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Separation ScienceCiting Articles
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This article has been cited by 3 ACS Journal articles (3 most recent appear below).
Analysis and Identification of Acid–Base Indicator Dyes by Thin-Layer Chromatography
Daniel D. ClarkJournal of Chemical Education2007 84 (7), 1186Analysis and Identification of Acid–Base Indicator Dyes by Thin-Layer Chromatography
Daniel D. ClarkJournal of Chemical Education2007 84 (7), 1186Thin-layer chromatography (TLC) is a simple and useful technique for the separation of compounds in a mixture. Organic chemists commonly employ TLC to follow the progression of a reaction, to qualitatively analyze a compound or mixture, to determine ...
The Preparation of a Fluorescent Dye: A Microscale TLC Experiment
Daniel S. Calimente , Shannon M. Strand , Shao-Chieh Chang and David E. LewisJournal of Chemical Education1999 76 (1), 82The Preparation of a Fluorescent Dye: A Microscale TLC Experiment
Daniel S. Calimente , Shannon M. Strand , Shao-Chieh Chang and David E. LewisJournal of Chemical Education1999 76 (1), 82This paper describes an experiment that allows the instructor to introduce TLC as a technique for analyzing reaction mixtures by monitoring the formation of a fluorescent dye. The experiment also illustrates the effects of eluting solvent on elution ...
A Simplified Method for the Microscale Extraction of Pigments from Spinach
Kimberley R. Cousins and Kathleen M. PiersonJournal of Chemical Education1998 75 (10), 1268A Simplified Method for the Microscale Extraction of Pigments from Spinach
Kimberley R. Cousins and Kathleen M. PiersonJournal of Chemical Education1998 75 (10), 1268A method is presented for microscale sample preparation for the thin-layer chromatographic analysis of the pigments in spinach. A commercial vegetable juicer is used for the initial extraction from spinach. This is followed by filtration, liquid/liquid ...
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- Received: August 03, 2009
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