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Separation of Enantiomeric Barbiturates by Capillary Electrophoresis Using a Cyclodextrin Containing Run Buffer
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Abstract
In the last few years capillary electrophoresis (CE) was successfully applied to a variety of analytical problems in the field of chiral separations. This article is intended to demonstrate the potential of CE for the determination of enantiomers. The separation is based on the addition of a suitable chiral selector to the buffer. Cyclodextrins perform as excellent selectors forming diastereomeric complexes of different stability with the enantiomeric forms of the analyte.
Barbiturates were chosen for chiral electrophoretic separation. They are found in a variety of pharmaceuticals such as sedatives, hypnotics and antiepileptics and are mostly processed as racemic mixtures. Cyclodextrins were applied as chiral selectors. The complex formation depends essentially on the structure of the selector and the analyte, this fact is discussed extensively in the paper. The influence of pH, the type and the concentration of the chiral selector on the separation were investigated.
The described experiment offers students an introduction to chiral separations by CE. The simple and fast method development along with the potential to solve difficult analytical problems make CE very attractive.
Keywords (Audience):
Second-Year UndergraduateKeywords (Domain):
Analytical ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Drugs / PharmaceuticalsCiting Articles
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This article has been cited by 11 ACS Journal articles (5 most recent appear below).
Capillary Electrophoresis: Focus on Undergraduate Laboratory Experiments
Lisa A. HollandJournal of Chemical Education2011 88 (3), 254-256Capillary Electrophoresis: Focus on Undergraduate Laboratory Experiments
Lisa A. HollandJournal of Chemical Education2011 88 (3), 254-256Capillary electrophoresis is a versatile technique well suited to teach concepts that are fundamental to a chemistry degree program. The applicability of capillary electrophoresis to metabolites, DNA, proteins, pharmaceutical compounds, environmental ...
Weak Acid pKa Determination Using Capillary Zone Electrophoresis
Mike SolowJournal of Chemical Education2006 83 (8), 1194Weak Acid pKa Determination Using Capillary Zone Electrophoresis
Mike SolowJournal of Chemical Education2006 83 (8), 1194This article describes a general chemistry experiment designed to measure the pKa of benzoic acid using capillary zone electrophoresis. Despite the fact that modern biology relies so heavily on this analytical technique, students get very little ...
Capillary Electrophoresis Analysis of Cations in Water Samples. An Experiment for the Introductory Laboratory
Christopher J. Pursell , Bert Chandler and Michelle M. BusheyJournal of Chemical Education2004 81 (12), 1783Capillary Electrophoresis Analysis of Cations in Water Samples. An Experiment for the Introductory Laboratory
Christopher J. Pursell , Bert Chandler and Michelle M. BusheyJournal of Chemical Education2004 81 (12), 1783Capillary electrophoresis is gradually working its way into the undergraduate laboratory curriculum. Typically, experiments utilizing this newer technology have been introduced into analytical or instrumental courses. We have introduced an experiment into ...
Determination of a Diffusion Coefficient by Capillary Electrophoresis. An Experiment for the Physical and Biophysical Chemistry Laboratories
Kathryn R. Williams , Bhavin Adhyaru , Igor German and Thomas RussellJournal of Chemical Education2002 79 (12), 1475Determination of a Diffusion Coefficient by Capillary Electrophoresis. An Experiment for the Physical and Biophysical Chemistry Laboratories
Kathryn R. Williams , Bhavin Adhyaru , Igor German and Thomas RussellJournal of Chemical Education2002 79 (12), 1475Students measure the diffusion coefficient of dopamine (3-hydroxytyramine) by capillary electrophoresis using three evaluation methods: single-point, graphical, and stopped migration. The experiment combines instruction of modern instrumentation with the ...
The Escalator—An Analogy for Explaining Electroosmotic Flow
Andrew J. Vetter and Garrett J. McGowanJournal of Chemical Education2001 78 (2), 209The Escalator—An Analogy for Explaining Electroosmotic Flow
Andrew J. Vetter and Garrett J. McGowanJournal of Chemical Education2001 78 (2), 209Capillary electrophoresis (CE) is an instrumental technique that is rapidly gaining popularity for use in the undergraduate laboratory. As a result, it becomes necessary to integrate the topic of CE into the junior/senior-level instrumental analysis ...
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- Received: August 03, 2009
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