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Dry-Column Flash Chromatography
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Abstract
Dry-column flash chromatography is a safe, powerful, yet easily learned preparative chromatography technique. It has proven useful in research, and an adaptation of the technique for use in large teaching laboratories (general chemistry, organic chemistry) is described here. The student version is similar to vacuum filtration, uses the same compact, readily available glassware, and inexpensive and safe solvents (ethyl acetate and hexane) and adsorbent (Merck grade 60 silica gel). The technique is sufficiently simple and powerful that a beginning student can successfully resolve diastereomers on sample scales ranging from 100 mg to >1 g.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Laboratory InstructionKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Laboratory Equipment / ApparatusCiting Articles
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This article has been cited by 6 ACS Journal articles (5 most recent appear below).
Using Artificial Soil and Dry-Column Flash Chromatography To Simulate Organic Substance Leaching Process: A Colorful Environmental Chemistry Experiment
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Isa G. J. de Avellar, Taís A. P. G. Cotta, and Amarílis de V. Finageiv NederJournal of Chemical Education2012 89 (2), 248-253Soil is an important and complex environmental compartment and soil contamination contributes to the pollution of aquifers and other water basins. A simple and low-cost experiment is described in which the mobility of three organic compounds in an ...
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Gail HorowitzJournal of Chemical Education2007 84 (2), 346This review explores the dramatic changes that have taken place in the organic chemistry laboratory course over the last two to three decades. The most significant changes have been in the areas of pedagogy and technology. Significant inroads have been ...
Anatomy of a Gel. Amino Acid Derivatives That Rigidify Water at Submillimolar Concentrations
Fredric M. Menger and Kevin L. CaranJournal of the American Chemical Society2000 122 (47), 11679-11691Anatomy of a Gel. Amino Acid Derivatives That Rigidify Water at Submillimolar Concentrations
Fredric M. Menger and Kevin L. CaranJournal of the American Chemical Society2000 122 (47), 11679-11691On the basis of suggestive X-ray data, 14 aroyl l-cystine derivatives were designed, synthesized, and examined for their ability to gelate water. Several members of this amino acid family are remarkably effective aqueous gelators (the best being one that ...
Undergraduate Separations Utilizing Flash Chromatography
Gail HorowitzJournal of Chemical Education2000 77 (2), 263Undergraduate Separations Utilizing Flash Chromatography
Gail HorowitzJournal of Chemical Education2000 77 (2), 263This article describes the procedures used to carry out four flash chromatography experiments: the isolation of the carotenes, chlorophylls and xanthophylls from a spinach extract; the separation of ß-carotene from tetraphenyl cyclopentadienone; the ...
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- Received: August 03, 2009
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