Article

Dry-Column Flash Chromatography

Reed College, Department of Chemistry, 3203 S. E. Woodstock Blvd., Portland, OR 97202-8199
J. Chem. Educ., 1997, 74 (10), p 1222
DOI: 10.1021/ed074p1222
Publication Date (Web): October 1, 1997

Abstract

Dry-column flash chromatography is a safe, powerful, yet easily learned preparative chromatography technique. It has proven useful in research, and an adaptation of the technique for use in large teaching laboratories (general chemistry, organic chemistry) is described here. The student version is similar to vacuum filtration, uses the same compact, readily available glassware, and inexpensive and safe solvents (ethyl acetate and hexane) and adsorbent (Merck grade 60 silica gel). The technique is sufficiently simple and powerful that a beginning student can successfully resolve diastereomers on sample scales ranging from 100 mg to >1 g.

Keywords (Audience):

Upper-Division Undergraduate;

Keywords (Domain):

Laboratory Instruction;

Keywords (Pedagogy):

Hands-On Learning / Manipulatives;

Keywords (Subject):

Laboratory Equipment / Apparatus;

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Article Views: 1,524 Times
Received 3 August 2009
Published online 1 October 1997
Published in print 1 October 1997
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