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Disadvantages of Double Reciprocal Plots

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R. Bruce Martin

University of Virginia, Chemistry Department, McCormick Road, Charlottesville, VA 22901

J. Chem. Educ., 1997, 74 (10), p 1238

DOI: 10.1021/ed074p1238

Publication Date (Web): October 1, 1997

Section:

History, Education, and Documentation

Abstract

Because they involve grossly uneven weightings of points, the linear and formally similar double reciprocal Benesi-Hildebrand and Lineweaver-Burke plots should never be used to resolve equilibrium and enzyme kinetic results. In such plots weightings from one end to the other may vary as much as a thousand or more. More equal weightings of the experimental points may be obtained by utilizing the linear, single reciprocal Scott and Hanes plots. The most appropriate resolution is achieved by a non-linear least squares analysis with the most error prone observable expressed as a function of all other variables and parameters.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Analytical Chemistry

Keywords (Subject):

Biophysical Chemistry

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