Photosensitized Peroxidation of Lipids: An Experiment Using 1H-NMR

Marion W. Smith , Renee Brown , Steven Smullin and Jon Eager
Muhlenberg College, Department of Chemistry, 2400 Chew Street, Allentown, PA 18104-5586
J. Chem. Educ., 1997, 74 (12), p 1471
DOI: 10.1021/ed074p1471
Publication Date (Web): December 1, 1997
History, Education, and Documentation

Abstract

The photoperoxidation of methyl linoleate, using 5,10,15,20-tetraphenyl porphyrin as photosensitizer, was monitored by 60 MHz 1H-NMR. Samples were irradiated for 10-24 hours in front of a 15 W fluorescent light, and NMR signals in the 5-6 ppm and 10-11 ppm region of the spectrum indicated peroxidation products were formed. The absorption of oxygen from the air was measured by attaching the sample tube to a gas burette. When vitamin E was added to the mixture the extent of peroxidation was reduced, showing the protective effect of the antioxidant. These experiments are appropriate for students of biochemistry

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Laboratory Instruction

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Instrumental Methods
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  • Received: August 03, 2009

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