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A Laboratory Experiment Investigating Different Aspects of Catalase Activity in an Inquiry - Based Approach
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Abstract
The action of the enzyme catalase on aqueous hydrogen peroxide to generate oxygen gas is a well-established demonstration. Catalase is typically obtained by aqueous extraction of a potato, and the potato extract is mixed together with 3% hydrogen peroxide. The oxygen that is produced can be collected over water. Variations on the procedure can demonstrate the dependence of catalytic activity on temperature or the presence of inhibitors.
Keywords (Audience):
High School / Introductory ChemistryKeywords (Domain):
Laboratory InstructionKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
CatalysisCiting Articles
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This article has been cited by 8 ACS Journal articles (5 most recent appear below).
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Suparna Chatterjee , Vickie M. Williamson , Kathleen McCann and M. Larry PeckJournal of Chemical Education2009 86 (12), 1427Inquiry-based laboratory experiments can play an important role for students in developing concepts and promoting deeper understanding. This study examined students' ability to differentiate between guided- and open-inquiry laboratories near the end of a ...
A Quick and Accurate Oxygen-Based Pressure-Sensor Assay for Catalase Activity
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Megan E. Lewis , Rebecca M. Levine , John T. York and W. Tandy GrubbsJournal of Chemical Education2009 86 (10), 1227A method is described for investigating the catalytic decomposition of hydrogen peroxide by using a computer-interfaced pressure sensor to follow the evolution of O2(g) inside a closed reaction vessel. The pressure sensor and accompanying glassware are ...
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John H. Penn and Abdulrahman G. Al-ShammariJournal of Chemical Education2008 85 (9), 1291A new method for teaching organic reaction mechanisms that can be used in a computer-assisted Instruction (CAI) environment is proposed and tested. The method concentrates on the intermediate structures that are assumed to be on the reaction coordinate ...
An Enzyme Kinetics Experiment Using Laccase for General Chemistry
Yaqi Lin and Patrick M. LloydJournal of Chemical Education2006 83 (4), 638An Enzyme Kinetics Experiment Using Laccase for General Chemistry
Yaqi Lin and Patrick M. LloydJournal of Chemical Education2006 83 (4), 638This article describes the use of laccase, an oxidoreductase enzyme, to study the effects of enzyme catalysts on reaction rates. The experiment is intended for students in the second semester of the general chemistry sequence. The effects of environmental ...
Student Opinions Regarding Inquiry-Based Labs
Kelly Morgan DetersJournal of Chemical Education2005 82 (8), 1178Student Opinions Regarding Inquiry-Based Labs
Kelly Morgan DetersJournal of Chemical Education2005 82 (8), 1178A survey of high school chemistry teachers in the U.S. indicated that 45.5% of these teachers are not using inquiry methods in their classroom. Perhaps they would be persuaded by student insights into learning with inquiry. This article describes the ...
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- Received: August 03, 2009
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