A Laboratory Experiment Investigating Different Aspects of Catalase Activity in an Inquiry - Based Approach

Doris R. Kimbrough , Mary Ann Magoun and Meg Langfur
Department of Chemistry, Box 194, University of Colorado at Denver, PO Box 173364, Denver, CO 80217-3364
J. Chem. Educ., 1997, 74 (2), p 210
DOI: 10.1021/ed074p210
Publication Date (Web): February 1, 1997

Abstract

The action of the enzyme catalase on aqueous hydrogen peroxide to generate oxygen gas is a well-established demonstration. Catalase is typically obtained by aqueous extraction of a potato, and the potato extract is mixed together with 3% hydrogen peroxide. The oxygen that is produced can be collected over water. Variations on the procedure can demonstrate the dependence of catalytic activity on temperature or the presence of inhibitors.

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History

  • Received: August 03, 2009

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