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Simulation and Analysis of Differential Scanning Calorimetry Output: Protein Unfolding Studies
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Abstract
High sensitivity differential scanning calorimetry ( HSDSC ) is a powerful and increasingly used technique for the study of molecular energetics in relation to biopolymers (e.g. proteins and nucleic acids ) or biomacromolecular assemblies ( e.g. liposomes ). HSDSC is, for example, the only method which allows thermodynamic parameters to be obtained directly for protein unfolding.The thermodynamic basis for HSDSC signals obtained for the equilibrium unfolding of proteins as well as the formalism for generating simulations of HSDSC outputs for protein unfolding are discussed. This will allow students ( studying biophysical chemistry, biochemistry, biophysics, molecular biology and allied subjects at undergraduate and postgraduate level ) to obtain an understanding of the type of data analysis required for HSDSC experiments on proteins.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
BiochemistryKeywords (Subject):
Calorimetry / ThermochemistryCiting Articles
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This article has been cited by 6 ACS Journal articles (5 most recent appear below).
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Sergio Alberto Dassie , María Soledad Celej and Gerardo Daniel FidelioJournal of Chemical Education2005 82 (1), 85The aim of this work is to present the physicochemical basis underlying the changes in protein thermostability upon ligand binding. The article is addressed to advanced undergraduate and postgraduate chemistry students with an interest in protein ...
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J. C. W. Folmer and Stefan FranzenJournal of Chemical Education2003 80 (7), 813Study of Polymer Glasses by Modulated Differential Scanning Calorimetry in the Undergraduate Physical Chemistry Laboratory
J. C. W. Folmer and Stefan FranzenJournal of Chemical Education2003 80 (7), 813Recent technological advances in thermal analysis present educational opportunities. In particular, modulated differential scanning calorimetry (MDSC) can be used to contrast reversing and nonreversing processes in practical laboratory experiments. The ...
Using Infrared Spectroscopy to Investigate Protein Structure
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- Received: August 03, 2009
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