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Utilizing Isolation, Purification, and Characterization of Enzymes as Project-Oriented Labs for Undergraduate Biochemistry
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Abstract
Senior-level biochemistry labs are mostly verification-type laboratories with little chance for exploration. We have developed a project-based biochemistry laboratory which gives them a chance to carry out a major biochemistry project. In the first quarter it is based on the purification of the enzyme lysozyme. The students are given some basic information, and then work out the details of their own procedures, make up their own solutions, and work at their own pace. Students use centrifugation, ion-exchange chromatography, spectral enzyme assays, and SDS-gel electrophoresis to purify and characterize the protein. In the second quarter students are given acid phosphatase and the basic assay for the enzyme, and then develop and carry out a method for determining the kinetic parameters of the enzyme. These experiments continue the development of laboratory independence of the students which steadily progresses in most curriculum
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
BiochemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Separation ScienceCiting Articles
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This article has been cited by 9 ACS Journal articles (5 most recent appear below).
Teaching Protein Purification and Characterization Techniques. A Student-Initiated, Project-Oriented Biochemistry Laboratory Course
Gina MacDonaldJournal of Chemical Education2008 85 (9), 1250Teaching Protein Purification and Characterization Techniques. A Student-Initiated, Project-Oriented Biochemistry Laboratory Course
Gina MacDonaldJournal of Chemical Education2008 85 (9), 1250This report describes a biochemistry laboratory that is completely project-oriented. Upper-level biology and chemistry majors work in teams to purify a protein of their choice. After the student groups have completed literature searches, ordered reagents, ...
Cytochrome c: A Biochemistry Laboratory Course
John B. Vincent and Stephen A. WoskiJournal of Chemical Education2005 82 (8), 1211Cytochrome c: A Biochemistry Laboratory Course
John B. Vincent and Stephen A. WoskiJournal of Chemical Education2005 82 (8), 1211A unified biochemistry laboratory course has been developed. Rather than using miscellaneous laboratory experiments, the concept behind this course is to focus on a theme: the process of biochemical research. The laboratory deals entirely with one protein,...
A Novel and Innovative Biochemistry Laboratory: Crystal Growth of Hen Egg White Lysozyme
Elizabeth Garrett , Audrey Wehr , Rebecca Hedge , David L. Roberts and Jacqueline R. RobertsJournal of Chemical Education2002 79 (3), 366A Novel and Innovative Biochemistry Laboratory: Crystal Growth of Hen Egg White Lysozyme
Elizabeth Garrett , Audrey Wehr , Rebecca Hedge , David L. Roberts and Jacqueline R. RobertsJournal of Chemical Education2002 79 (3), 366Protein crystallization is the first and perhaps most important step in solving the structure of a protein via X-ray crystallography. However, very little time, if any, is spent working on this during laboratory experiments in undergraduate biochemistry ...
Using Infrared Spectroscopy to Investigate Protein Structure
Janet Olchowicz , Deidra R. Coles , Lori E. Kain and Gina MacDonaldJournal of Chemical Education2002 79 (3), 369Using Infrared Spectroscopy to Investigate Protein Structure
Janet Olchowicz , Deidra R. Coles , Lori E. Kain and Gina MacDonaldJournal of Chemical Education2002 79 (3), 369This report describes a laboratory that employs infrared spectroscopy to observe protein secondary structure. The techniques utilized are suitable for all levels of biochemistry or biophysical chemistry laboratories. Students first obtain absorbance ...
Deaf Students, Teachers, and Interpreters in the Chemistry Lab
Gina MacDonald , Brenda C. Seal , Dorothy H. WynneJournal of Chemical Education2002 79 (2), 239Deaf Students, Teachers, and Interpreters in the Chemistry Lab
Gina MacDonald , Brenda C. Seal , Dorothy H. WynneJournal of Chemical Education2002 79 (2), 239This report describes an undergraduate research program at James Madison University that includes deaf and hard-of-hearing students from Gallaudet University, deaf teachers from schools for the Deaf, and both professional interpreters and students engaged ...
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- Received: August 03, 2009
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