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HPLC for Undergraduate Introductory Laboratories

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Scott A. Van Arman and Marcus W. Thomsen

Department of Chemistry, Franklin and Marshall College, Lancaster, PA 17604

J. Chem. Educ., 1997, 74 (1), p 49

DOI: 10.1021/ed074p49

Publication Date (Web): January 1, 1997

Section:

History, Education, and Documentation

Abstract

Undergraduate laboratories continue increasing the use of instrumentation in teaching. One technique that is growing in popularity is HPLC. We have designed a set of simple HPLC separations as part of an introductory set of projects that serve as an introduction to chromatography early in the organic course. We have introduced quantitative analysis to the common separation of analgesics so that students may identify the composition of an unknown commercial tablet. Derived from this system is an adaptation of the well known separation of nucleosides by reversed-phase HPLC such that students can quantitatively identify the components of an unknown "RNA digest." Students must determine retention times and an instrumental response factor for each component. For both separations all components elute in â—Š 6 min. and baseline separation is excellent. From the retention times of standard individual component samples the identity of each component in the sample can be ascertained. From the instrumental response factors of standard individual component samples the percent composition of each component can be calculated.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Organic Chemistry

Keywords (Feature):

Waters Symposium

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Chromatography

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

The Combined Effects of pH and Percent Methanol on the HPLC Separation of Benzoic Acid and Phenol
Seema M. Joseph and John A. Palasota
Journal of Chemical Education2001 78 (10), 1381
- The Combined Effects of pH and Percent Methanol on the HPLC Separation of Benzoic Acid and Phenol
  Seema M. Joseph and John A. Palasota
  Journal of Chemical Education2001 78 (10), 1381
  The effect of varying mobile-phase pH and percent methanol on the reversed-phase HPLC separation of benzoic acid and phenol is investigated. Both analytes are of interest owing to their classification as moderate environmental and health hazards. Baseline ...
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