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Dramatic Demonstration of Oxygen Sensing by Luminescence Quenching

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Kristi A. Kneas , Wenying Xu and J. N. Demas

Department of Chemistry, University of Virginia, Charlottesville, VA 22901

B. A. DeGraff

Department of Chemistry, James Madison University, Harrisonburg, VA 22807

J. Chem. Educ., 1997, 74 (6), p 696

DOI: 10.1021/ed074p696

Publication Date (Web): June 1, 1997

Section:

History, Education, and Documentation

Abstract

Luminescence-based oxygen sensors are of great interest, especially in biomedical areas, but explaining their operation to the inexperienced can be difficult. We describe a dramatic demonstration that illustrates the principles of oxygen sensors using luminescence quenching of a transition metal complex, either [Ru(bpy)₃]Cl₂, (bpy= 2,2'-bipyridine) or [Ru(Ph₂phen)₃]Cl₂, (Ph₂phen = 4,7-diphenyl-1,10-phenanthroline). The demonstration is simple, quick, and comprehensible even by non-chemists. It requires three solutions of the Ru(II) complex, a black light, oxygen, and some dry ice. One solution is air saturated and two are oxygen saturated. The visual differences in emission intensity for the different oxygen concentrations are easily discernible even to a large audience. By dropping a piece of dry ice into one of the oxygen saturated solutions, the oxygen is removed in seconds, and a spectacular increase in emission intensity can be observed. Students and faculty alike find this a clever demonstration of quenching and its analytical applications.

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Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Inorganic Chemistry

Keywords (Feature):

Symposium Report

Keywords (Subject):

Oxygen

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This article has been cited by 2 ACS Journal articles (2 most recent appear below).

Use of a Long-Lifetime Re(I) Complex in Fluorescence Polarization Immunoassays of High-Molecular-Weight Analytes
Xiang-Qun Guo, Felix N. Castellano, Li Li, and Joseph R. Lakowicz
Analytical Chemistry1998 70 (3), 632-637
- Use of a Long-Lifetime Re(I) Complex in Fluorescence Polarization Immunoassays of High-Molecular-Weight Analytes
  Xiang-Qun Guo, Felix N. Castellano, Li Li, and Joseph R. Lakowicz
  Analytical Chemistry1998 70 (3), 632-637
  We describe a new class of fluorescence polarization immunoassays based on the luminescence from a Re(I) metal−ligand complex. Re(I) complexes are extremely photostable and possess useful photophysical properties including long lifetimes, high quantum ...
  Abstract | Full Text HTML | Hi-Res PDF
Applications of Luminescent Transition Metal Complexes to Sensor Technology and Molecular Probes
J. N. Demas , B. A. DeGraff
Journal of Chemical Education1997 74 (6), 690
- Applications of Luminescent Transition Metal Complexes to Sensor Technology and Molecular Probes
  J. N. Demas , B. A. DeGraff
  Journal of Chemical Education1997 74 (6), 690
  An overview of the applications of luminescent transition metal complexes to sensor technology is presented. In addition to general considerations, a number of specific systems are discussed including oxygen, pH, carbon dioxide, temperature, and ...
  Abstract | Hi-Res PDF | PDF w/ Links