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Automated Protein Assay Using Flow Injection Analysis

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Carrie A. C. Wolfe , Matthew R. Oates and David S. Hage

Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304

J. Chem. Educ., 1998, 75 (8), p 1025

DOI: 10.1021/ed075p1025

Publication Date (Web): August 1, 1998

Section:

History, Education, and Documentation

Abstract

The technique of flow injection analysis (FIA) is a common instrumental method used in detecting a variety of chemical and biological agents. This paper describes an undergraduate laboratory that uses FIA to perform a bicinchoninic acid (BCA) colorimetric assay for quantitating protein samples. The method requires less than 2 min per sample injection and gives a response over a broad range of protein concentrations. This method can be used in instrumental analysis labs to illustrate the principles and use of FIA, or as a means for introducing students to common methods employed in the analysis of biological agents.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Analytical Chemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Instrumental Methods

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This article has been cited by 3 ACS Journal articles (3 most recent appear below).

Automated Combinatorial Chemistry in the Organic Chemistry Majors Laboratory
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Journal of Chemical Education2010 87 (1), 87-90
- Automated Combinatorial Chemistry in the Organic Chemistry Majors Laboratory
  Christopher J. Nichols, Larry F. Hanne
  Journal of Chemical Education2010 87 (1), 87-90
  A multidisciplinary experiment has been developed in which students each synthesize a combinatorial library of 48 hydrazones with the aid of a liquid-handling robot. Each product is then subjected to a Kirby−Bauer disk diffusion assay to assess its ...
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Determination of Acidity Constants by Gradient Flow-Injection Titration
Manuel E. Minas da Piedade , António C. L. Conceição
Journal of Chemical Education2006 83 (12), 1853
- Determination of Acidity Constants by Gradient Flow-Injection Titration
  Manuel E. Minas da Piedade , António C. L. Conceição
  Journal of Chemical Education2006 83 (12), 1853
  The use of the gradient chamber flow-injection titration method (GCFIT) to determine acidity constants is illustrated through an experiment involving acetic and benzoic acids. The procedure relies on spectrophotometric detection and on the use of an acid–...
  Abstract | Hi-Res PDF | PDF w/ Links
Determination of Hypochlorite in Bleaching Products with Flower Extracts To Demonstrate the Principles of Flow Injection Analysis
Luiz Antônio Ramos , Kátia Roberta Prieto , Éder Tadeu Gomes Cavalheiro and Carla Cristina Schmitt Cavalheiro
Journal of Chemical Education2005 82 (12), 1815
- Determination of Hypochlorite in Bleaching Products with Flower Extracts To Demonstrate the Principles of Flow Injection Analysis
  Luiz Antônio Ramos , Kátia Roberta Prieto , Éder Tadeu Gomes Cavalheiro and Carla Cristina Schmitt Cavalheiro
  Journal of Chemical Education2005 82 (12), 1815
  The use of crude flower extracts is proposed to teach the principles of flow injection procedures. In this experiment a bleaching reaction between hypochlorite and the anthocyanins contained in the flower extracts is examined. The bleaching of the crude ...
  Abstract | Hi-Res PDF | PDF w/ Links