Capillary Electrophoresis in the Analytical and Physical Chemistry Laboratories

Kathryn R. Williams
University of Florida, Department of Chemistry, P.O. Box 117200, Gainesville, FL 32611-7200
J. Chem. Educ., 1998, 75 (9), p 1079
DOI: 10.1021/ed075p1079
Publication Date (Web): September 1, 1998

Abstract

Availability of capillary electrophoresis (CE) instrumentation allows students to have hands-on experience with this modern separation tool. They gain more than operational knowledge, however. Inherent in the instructional process is an emphasis on the relation of the chemistry of the sample to the fundamental mechanism of electrophoresis and the demonstration of how CE complements other methods for similar measurements.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Physical Chemistry

Keywords (Feature):

NSF Highlights

Keywords (Subject):

Separation Science

Citing Articles

View all 5 citing articles

Citation data is made available by participants in CrossRef's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search in SciFinder.

This article has been cited by 5 ACS Journal articles (5 most recent appear below).

  • Cover Image

    Microfluidic Gel Electrophoresis in the Undergraduate Laboratory Applied to Food Analysis

    Tzu-Chiao Chao, Sanchari Bhattacharya, and Alexandra Ros
    Journal of Chemical Education2012 89 (1), 125-129
    • Microfluidic Gel Electrophoresis in the Undergraduate Laboratory Applied to Food Analysis

      Tzu-Chiao Chao, Sanchari Bhattacharya, and Alexandra Ros
      Journal of Chemical Education2012 89 (1), 125-129

      A microfluidics-based laboratory experiment for the analysis of DNA fragments in an analytical undergraduate course is presented. The experiment is set within the context of food species identification via amplified DNA fragments. The students are ...

  • Cover Image

    Presentation and Impact of Experimental Techniques in Chemistry

    Zbigniew Sojka , Michel Che
    Journal of Chemical Education2008 85 (7), 934
    • Presentation and Impact of Experimental Techniques in Chemistry

      Zbigniew Sojka , Michel Che
      Journal of Chemical Education2008 85 (7), 934

      Laboratory and practical courses, where students become familiar with experimental techniques and learn to interpret data and relate them to appropriate theory, play a vital role in chemical education. In the large panoply of currently available ...

  • Cover Image

    Weak Acid pKa Determination Using Capillary Zone Electrophoresis

    Mike Solow
    Journal of Chemical Education2006 83 (8), 1194
    • Weak Acid pKa Determination Using Capillary Zone Electrophoresis

      Mike Solow
      Journal of Chemical Education2006 83 (8), 1194

      This article describes a general chemistry experiment designed to measure the pKa of benzoic acid using capillary zone electrophoresis. Despite the fact that modern biology relies so heavily on this analytical technique, students get very little ...

  • Cover Image

    Determination of a Diffusion Coefficient by Capillary Electrophoresis. An Experiment for the Physical and Biophysical Chemistry Laboratories

    Kathryn R. Williams , Bhavin Adhyaru , Igor German and Thomas Russell
    Journal of Chemical Education2002 79 (12), 1475
    • Determination of a Diffusion Coefficient by Capillary Electrophoresis. An Experiment for the Physical and Biophysical Chemistry Laboratories

      Kathryn R. Williams , Bhavin Adhyaru , Igor German and Thomas Russell
      Journal of Chemical Education2002 79 (12), 1475

      Students measure the diffusion coefficient of dopamine (3-hydroxytyramine) by capillary electrophoresis using three evaluation methods: single-point, graphical, and stopped migration. The experiment combines instruction of modern instrumentation with the ...

  • Cover Image

    The Escalator—An Analogy for Explaining Electroosmotic Flow

    Andrew J. Vetter and Garrett J. McGowan
    Journal of Chemical Education2001 78 (2), 209
    • The Escalator—An Analogy for Explaining Electroosmotic Flow

      Andrew J. Vetter and Garrett J. McGowan
      Journal of Chemical Education2001 78 (2), 209

      Capillary electrophoresis (CE) is an instrumental technique that is rapidly gaining popularity for use in the undergraduate laboratory. As a result, it becomes necessary to integrate the topic of CE into the junior/senior-level instrumental analysis ...

Tools

SciFinder Links

SciFinder subscribers:  Click to sign in | Not a SciFinder subscriber? Learn more at www.cas.org

Explore by:


History

  • Received: August 03, 2009

Recommend & Share

  • Share on ACS NetworkACS Network
  • Add to FacebookFacebook
  • Tweet ThisTweet This
  • Add to CiteULikeCiteULike
  • Add to NewsvineNewsvine
  • Digg ThisDigg This
  • Add to DeliciousDelicious

Related Content