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A Simplified Method for the Microscale Extraction of Pigments from Spinach

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Kimberley R. Cousins and Kathleen M. Pierson

Department of Chemistry, California State University, San Bernardino, San Bernardino, CA 92407

J. Chem. Educ., 1998, 75 (10), p 1268

DOI: 10.1021/ed075p1268

Publication Date (Web): October 1, 1998

Section:

History, Education, and Documentation

Abstract

A method is presented for microscale sample preparation for the thin-layer chromatographic analysis of the pigments in spinach. A commercial vegetable juicer is used for the initial extraction from spinach. This is followed by filtration, liquid/liquid extraction, centrifugation, and evaporation. The new sample preparation technique is significantly more efficient than present methods and minimizes solvents used for extraction.

Keywords (Audience):

First-Year Undergraduate / General

Keywords (Domain):

Laboratory Instruction

Keywords (Feature):

The Microscale Laboratory

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Chromatography

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This article has been cited by 3 ACS Journal articles (3 most recent appear below).

Research Advances: Spinach Nutrient Levels; Steering Oil Droplets; Possible Explanation of Left-Handed Preference?
Angela G. King
Journal of Chemical Education2010 87 (8), 766-769
- Research Advances: Spinach Nutrient Levels; Steering Oil Droplets; Possible Explanation of Left-Handed Preference?
  Angela G. King
  Journal of Chemical Education2010 87 (8), 766-769
  This installment of Research Advances describes investigations of how supermarket storage affects spinach nutrient levels, working on oil droplets that can navigate a maze, and an explanation of nature’s selection for l-amino acids.
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
The Garden of Green Organic Chemistry at Hendrix College
Thomas E. Goodwin
2009 1011 (), 37-53
- The Garden of Green Organic Chemistry at Hendrix College
  Thomas E. Goodwin
  2009 1011 (), 37-53
  The Hendrix College organic chemistry laboratories were converted to microscale experiments in 1988 to minimize possible adverse environmental impact, increase lab safety, and decrease generation of waste and costs of waste disposal. As we became aware of ...
  Abstract | Hi-Res PDF | PDF w/ Links
An Improved Method for the Extraction and Thin-Layer Chromatography of Chlorophyll a and b from Spinach
Hao T. Quach , Robert L. Steeper and G. William Griffin
Journal of Chemical Education2004 81 (3), 385
- An Improved Method for the Extraction and Thin-Layer Chromatography of Chlorophyll a and b from Spinach
  Hao T. Quach , Robert L. Steeper and G. William Griffin
  Journal of Chemical Education2004 81 (3), 385
  A direct method of extracting plant pigments from spinach leaves into a dry organic solvent is presented. A powder is obtained by grinding a mixture of raw spinach, drying agent, and sand (1:1:2) that can be extracted directly into acetone in 10 minutes ...
  Abstract | Hi-Res PDF | PDF w/ Links