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Enzyme-Linked Antibodies: A Laboratory Introduction to the ELISA Assay

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Gretchen L. Anderson and Leo A. McNellis

Department of Chemistry, Indiana University South Bend, South Bend, IN 46634

J. Chem. Educ., 1998, 75 (10), p 1275

DOI: 10.1021/ed075p1275

Publication Date (Web): October 1, 1998

Section:

History, Education, and Documentation

Abstract

A fast and economical laboratory exercise is presented that qualitatively demonstrates the power of enzyme-linked antibodies to detect a specific antigen. Although ELISAs are commonly used in disease diagnosis in clinical settings, this application uses biotin, covalently attached to albumin, to take advantage of readily available reagents and avoids problems associated with potentially pathogenic antigens. The laboratory exercise is suitable for high school or freshman level biochemistry courses, and can be completed within two hours.

Keywords (Audience):

High School / Introductory Chemistry

Keywords (Domain):

Biochemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Enzymes

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