Prev. Article Next Article Table of Contents

Article

Immobilized Lactase in the Biochemistry Laboratory

Citing Articles

Your current credentials do not allow retrieval of the full text.

Purchase the full-text

PDF/HTML,
figures/images,
references and tables,
(where available)

Matthew J. Allison and C. Larry Bering

Department of Chemistry, Clarion University, Clarion, PA 16214

J. Chem. Educ., 1998, 75 (10), p 1278

DOI: 10.1021/ed075p1278

Publication Date (Web): October 1, 1998

Section:

History, Education, and Documentation

Abstract

Immobilized enzymes have many practical applications. They may be used in clinical, industrial, and biotechnological laboratories and in many clinical diagnostic kits. For educational purposes, use of immobilized enzymes can easily be taught at the undergraduate or even secondary level. We have developed an immobilized enzyme experiment that combines many practical techniques used in the biochemistry laboratory and fits within a three-hour time frame. In this experiment, lactase from over-the-counter tablets for patients with lactose intolerance is immobilized in polyacrylamide, which is then milled into small beads and placed into a chromatography column. A lactose solution is added to the column and the eluant is assayed using the glucose oxidase assay, available as a kit. We have determined the optimal conditions to give the greatest turnover of lactose while allowing the immobilized enzymes to be active for long periods at room temperature.

Keywords (Audience):

First-Year Undergraduate / General

Keywords (Domain):

Laboratory Instruction

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Enzymes

View: Hi-Res PDF | PDF w/ Links

Citing Articles

View all 2 citing articles

Citation data is made available by participants in CrossRef's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search in SciFinder.

This article has been cited by 2 ACS Journal articles (2 most recent appear below).

Biocatalysis with Sol−Gel Encapsulated Acid Phosphatase
Suhasini Kulkarni, Vu Tran, Maggie K.-M. Ho, Chieu Phan, Elizabeth Chin, Zeke Wemmer and Monika Sommerhalter
Journal of Chemical Education2010 87 (9), 958-960
- Biocatalysis with Sol−Gel Encapsulated Acid Phosphatase
  Suhasini Kulkarni, Vu Tran, Maggie K.-M. Ho, Chieu Phan, Elizabeth Chin, Zeke Wemmer and Monika Sommerhalter
  Journal of Chemical Education2010 87 (9), 958-960
  This experiment was performed in an upper-level undergraduate biochemistry laboratory course. Students learned how to immobilize an enzyme in a sol−gel matrix and how to perform and evaluate enzyme-activity measurements. The enzyme acid phosphatase (APase)...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
Immobilized alpha-Galactosidase in the Biochemistry Laboratory
V. H. Mulimani and K. Dhananjay
Journal of Chemical Education2007 84 (12), 1974
- Immobilized alpha-Galactosidase in the Biochemistry Laboratory
  V. H. Mulimani and K. Dhananjay
  Journal of Chemical Education2007 84 (12), 1974
  This laboratory experiment was designed to demonstrate the application of immobilized galactosidase in food industry to hydrolyze raffinose family oligosaccharides in soymilk. This laboratory experiment was conducted for postgraduate students of ...
  Abstract | Hi-Res PDF | PDF w/ Links