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A 19F NMR Study of Enzyme Activity

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Keith E. Peterman , Kevin Lentz and Jeffery Duncan

York College of Pennsylvania, Physical Sciences Department, York, PA 17405

J. Chem. Educ., 1998, 75 (10), p 1283

DOI: 10.1021/ed075p1283

Publication Date (Web): October 1, 1998

Section:

History, Education, and Documentation

Abstract

This basic enzyme activity laboratory experiment demonstrates how ¹⁹F NMR can be used in biochemical studies and presents the advantages of ¹⁹F NMR over ¹H NMR for studies of this nature. N-Trifluoroacetylglycine was selected as a commercially available model fluorine-tagged substrate that readily undergoes acylase I-catalyzed hydrolysis to produce trifluoroacetic acid and glycine. Progress of the reaction was monitored by following conversion of the trifluoroacetyl moiety peak of N-trifluoroacetylglycine to trifluoroacetic acid. The extent of hydrolysis was determined by comparing integrated ratios of the two ¹⁹F NMR peaks. A plot of percent hydrolysis versus enzyme concentration was used to calculate unit activity of the enzyme. This is a viable laboratory experiment for junior/senior-level courses in instrumental analytical chemistry, biochemistry, molecular biology, or spectroscopy.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Biochemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Fluorine

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

Toward the Integration of Liquid- and Solid-State NMR across the Undergraduate Curriculum
N. M. Washton, K. C. Earnheart, D. G. Sykes, M. Ucak-Astarlioglu, and K. T. Mueller
2007 969 (), 20-35
- Toward the Integration of Liquid- and Solid-State NMR across the Undergraduate Curriculum
  N. M. Washton, K. C. Earnheart, D. G. Sykes, M. Ucak-Astarlioglu, and K. T. Mueller
  2007 969 (), 20-35
  This work addresses issues of fundamental reform in the undergraduate chemistry curriculum via advanced integration of liquid- and solid-state nuclear magnetic resonance (NMR) experiments and theory. Our integrated curricula comprises a suite of NMR ...
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