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Determination of the Enantiomeric Purity of Commercial L-[U-14C] Valine: An Experiment Utilizing Reversed-Phase Thin-Layer Chromatography and Liquid Scintillation Counting
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Abstract
The enantiomeric purity of commercial L-[U-14C]valine was determined. The process involved sequential dilution with nonradioactive DL-valine, N-dansylation using dansyl chloride, and resolution using reversed-phase thin-layer chromatography in the presence of σ-cyclodextrin, a chiral mobile phase additive. Liquid scintillation counting of the bands corresponding to DNS-D- and L-valine gave the enantiomeric purity, as well as the percent radiochemical yield. Because the derivatization reaction proceeded without racemization, the results corresponded to the relative amounts of radioactive D- and L-valine in the commercial sample. The analyses were easily performed using less than 1 µ</Ι>Ci of L-[U-14C]valine per pair of students. This laboratory experiment provides students with valuable experience in handling radioisotopes, illustrates the use of liquid scintillation counting as a sensitive detection method, reinforces important stereochemical relationships, and can be applied to the analysis of other radioactive amino acids.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Laboratory InstructionKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
ChromatographyCiting Articles
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This article has been cited by 4 ACS Journal articles (4 most recent appear below).
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- Received: August 03, 2009
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