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Chromatography, Absorption, and Fluorescence: A New Instrumental Analysis Experiment on the Measurement of Polycyclic Aromatic Hydrocarbons in Cigarette Smoke
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Abstract
The recent approval by the American Chemical Society of an undergraduate chemistry degree with an option in environmental chemistry requires the development of new experiments that teach fundamental chemistry in the context of environmental issues. We present an experiment suitable for an undergraduate junior/senior-level instrumental analysis laboratory which illustrates the principles of high-performance liquid chromatography (HPLC) and its application to the identification and measurement of polycyclic aromatic hydrocarbons (PAH) in tobacco smoke. Absorption and fluorescence detection methods for PAH, especially the differences in sensitivity and selectivity of these methods, are clearly demonstrated along with the basic principles of HPLC.
Keywords (Audience):
Second-Year UndergraduateKeywords (Domain):
Environmental ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Instrumental MethodsCiting Articles
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This article has been cited by 5 ACS Journal articles (5 most recent appear below).
Fluorescence, Absorption, and Excitation Spectra of Polycyclic Aromatic Hydrocarbons as a Tool for Quantitative Analysis
A. M. Rivera-Figueroa , K. A. Ramazan and B. J. Finlayson-PittsJournal of Chemical Education2004 81 (2), 242Fluorescence, Absorption, and Excitation Spectra of Polycyclic Aromatic Hydrocarbons as a Tool for Quantitative Analysis
A. M. Rivera-Figueroa , K. A. Ramazan and B. J. Finlayson-PittsJournal of Chemical Education2004 81 (2), 242An experiment suitable for college junior or senior students in the analytical instrumental analysis laboratory that demonstrates the analysis of PAHs (benzo[a]anthracene, benzo[k]fluoranthene, benzo[a]pyrene, chrysene, and phenanthrene) using absorption ...
Measurement of Trace Metals in Tobacco and Cigarette Ash by Inductively Coupled Plasma-Atomic Emission Spectroscopy
W. Wang and B. J. Finlayson-PittsJournal of Chemical Education2003 80 (1), 83Measurement of Trace Metals in Tobacco and Cigarette Ash by Inductively Coupled Plasma-Atomic Emission Spectroscopy
W. Wang and B. J. Finlayson-PittsJournal of Chemical Education2003 80 (1), 83The ICP–AES experiment reported here is suitable for use in a junior- or senior-level undergraduate instrumental analysis laboratory. The objective of this experiment is to analyze trace metals present in cigarette tobacco, the cigarette filter, and the ...
How Does Light Scattering Affect Luminescence? Fluorescence Spectra and Quantum Yields in the Solid Phase
M. Gabriela Lagorio and Enrique San RománJournal of Chemical Education2002 79 (11), 1362How Does Light Scattering Affect Luminescence? Fluorescence Spectra and Quantum Yields in the Solid Phase
M. Gabriela Lagorio and Enrique San RománJournal of Chemical Education2002 79 (11), 1362Fluorescence quantum yields and luminescence spectra for solid samples are presented and interpreted. Re-absorption of the emitted light is very important in these systems causing a decrease in the observed luminescence quantum yield as well as ...
The Determination of Ergosterol in Environmental Samples. An Interdisciplinary Project Involving Techniques of Analytical and Organic Chemistry
Eugene J. Volker , Dan DiLella , Kim Terneus , Carson Baldwin and Ilona VolkerJournal of Chemical Education2000 77 (12), 1621The Determination of Ergosterol in Environmental Samples. An Interdisciplinary Project Involving Techniques of Analytical and Organic Chemistry
Eugene J. Volker , Dan DiLella , Kim Terneus , Carson Baldwin and Ilona VolkerJournal of Chemical Education2000 77 (12), 1621In this undergraduate laboratory experiment, a naturally formed steroid that is an important environmental indicator of biological activity is extracted and quantified. The target compound is ergosterol, a steroid found only in fungi and an accepted ...
ISE Analysis of Hydrogen Sulfide in Cigarette Smoke
Guofeng Li , Brian J. Polk , Liz A. Meazell and David W. HatchettJournal of Chemical Education2000 77 (8), 1049ISE Analysis of Hydrogen Sulfide in Cigarette Smoke
Guofeng Li , Brian J. Polk , Liz A. Meazell and David W. HatchettJournal of Chemical Education2000 77 (8), 1049Many advanced undergraduate analytical laboratory courses focus on exposing students to various modern instruments. However, students rarely have the opportunity to construct their own analytical tools for solving practical problems. We designed an ...
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- Received: August 03, 2009
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