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Capillary Electrophoresis: Part II. Applications
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Abstract
Since its advent, capillary electrophoresis (CE) has been used to solve many chemical separation problems. Isolation and identification of components in samples ranging from human cells to shale oil have been achieved using CE.
A wide variety of applications of CE have appeared in the literature. Many of these applications are presented in this article so as to demonstrate the power and versatility of CE. Separations of ionizable species including numerous small anions, proteins, and biopolymers are presented. Packed capillary and affinity CE methods are also discussed. Examples of the selectivity and resolving power of additives such as micelles and cyclodextrins are provided. Finally, the "future" of CE, including a examples of "CE on a chip," is discussed.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Analytical ChemistryKeywords (Feature):
Topics in Chemical InstrumentationKeywords (Subject):
Separation ScienceCiting Articles
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This article has been cited by 6 ACS Journal articles (5 most recent appear below).
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Mike SolowJournal of Chemical Education2006 83 (8), 1194Weak Acid pKa Determination Using Capillary Zone Electrophoresis
Mike SolowJournal of Chemical Education2006 83 (8), 1194This article describes a general chemistry experiment designed to measure the pKa of benzoic acid using capillary zone electrophoresis. Despite the fact that modern biology relies so heavily on this analytical technique, students get very little ...
Capillary Electrophoresis Analysis of Cations in Water Samples. An Experiment for the Introductory Laboratory
Christopher J. Pursell , Bert Chandler and Michelle M. BusheyJournal of Chemical Education2004 81 (12), 1783Capillary Electrophoresis Analysis of Cations in Water Samples. An Experiment for the Introductory Laboratory
Christopher J. Pursell , Bert Chandler and Michelle M. BusheyJournal of Chemical Education2004 81 (12), 1783Capillary electrophoresis is gradually working its way into the undergraduate laboratory curriculum. Typically, experiments utilizing this newer technology have been introduced into analytical or instrumental courses. We have introduced an experiment into ...
Microscale Capillary Electrophoresis: A Complete Instrumentation Experiment for Chemistry Students at the Undergraduate Junior or Senior Level
Ruben T. Almaraz , Maria KochisJournal of Chemical Education2003 80 (3), 316Microscale Capillary Electrophoresis: A Complete Instrumentation Experiment for Chemistry Students at the Undergraduate Junior or Senior Level
Ruben T. Almaraz , Maria KochisJournal of Chemical Education2003 80 (3), 316A microscale capillary electrophoresis (CE) system was created with conventional devices. Mechanical and thermal techniques were implemented in the creation of a microchip in a microscope glass slide for analyte separation. The detection system consisted ...
Determination of a Diffusion Coefficient by Capillary Electrophoresis. An Experiment for the Physical and Biophysical Chemistry Laboratories
Kathryn R. Williams , Bhavin Adhyaru , Igor German and Thomas RussellJournal of Chemical Education2002 79 (12), 1475Determination of a Diffusion Coefficient by Capillary Electrophoresis. An Experiment for the Physical and Biophysical Chemistry Laboratories
Kathryn R. Williams , Bhavin Adhyaru , Igor German and Thomas RussellJournal of Chemical Education2002 79 (12), 1475Students measure the diffusion coefficient of dopamine (3-hydroxytyramine) by capillary electrophoresis using three evaluation methods: single-point, graphical, and stopped migration. The experiment combines instruction of modern instrumentation with the ...
The Escalator—An Analogy for Explaining Electroosmotic Flow
Andrew J. Vetter and Garrett J. McGowanJournal of Chemical Education2001 78 (2), 209The Escalator—An Analogy for Explaining Electroosmotic Flow
Andrew J. Vetter and Garrett J. McGowanJournal of Chemical Education2001 78 (2), 209Capillary electrophoresis (CE) is an instrumental technique that is rapidly gaining popularity for use in the undergraduate laboratory. As a result, it becomes necessary to integrate the topic of CE into the junior/senior-level instrumental analysis ...
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- Received: August 03, 2009
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