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Use of a Spreadsheet To Simulate Enzyme Kinetics

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Michael F. Bruist

Philadelphia College of Science and Pharmacy, Department of Chemistry and Biochemistry, 600 S. 43rd Street, Philadelphia, PA 19104-4495

J. Chem. Educ., 1998, 75 (3), p 372

DOI: 10.1021/ed075p372

Publication Date (Web): March 1, 1998

Section:

History, Education, and Documentation

Abstract

A spreadsheet with graphics capabilities is used to model enzyme kinetics and to explain how computer simulation of a reaction mechanism is accomplished by the Gauss-Cauchey method. The exercises described help illuminate the link between chemical phenomena and their mathematical description. I use these exercises in an advanced undergraduate biochemistry course. They can be done in two or three 4-hour computer laboratory sessions. The first session introduces students to spreadsheets using a simple first order reaction, the decay of 14C. Enzyme kinetics are developed in the remaining sessions using the mythical enzyme Midase. The spread sheets can be modified to illustrate more complex schemes such as inhibition or initial burst kinetics.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Biochemistry

Keywords (Feature):

Computer Bulletin Board

Keywords (Pedagogy):

Computer-Based Learning

Keywords (Subject):

Enzymes

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Citing Articles

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This article has been cited by 11 ACS Journal articles (5 most recent appear below).

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  This article describes a comprehensive treatment of experimental enzyme kinetics strongly coupled to electronic data acquisition and use of spreadsheets to organize data and perform linear and nonlinear least-squares analyses, all in a manner that ...
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Journal of Chemical Education2008 85 (8), 1146
- A Convenient Tool for the Stochastic Simulation of Reaction Mechanisms
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  The article introduces the stochastic simulation of chemical reactions with Chemical Kinetics Simulator, CKS, a free, easy-to-use, and powerful program. After familiarizing themselves with the Monte Carlo method of stochastic simulation and CKS, students ...
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Journal of Chemical Education2006 83 (12), 1879
- Design of an Excel Spreadsheet To Estimate Rate Constants, Determine Associated Errors, and Choose Curve's Extent
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  In this article we wish to present a new Microsoft Excel spreadsheet design that enables a prompt calculation of rate constant values (k) and associated errors and also addresses less common features such as the choice of experimental curve's extent. The ...
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Kinetica: An Excel Program To Simulate or Analyze Kinetic Data
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Journal of Chemical Education2004 81 (1), 159
- Kinetica: An Excel Program To Simulate or Analyze Kinetic Data
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  Journal of Chemical Education2004 81 (1), 159
  Instructors or students can generate simulated kinetic data using parameters they specify, or using parameters randomized by Kinetica. The data set that is generated may then be exported for use in exercises, homework, and exams, or may be analyzed ...
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Peer-Reviewed JCE WebWare
William F. Coleman , Edward W. Fedosky
Journal of Chemical Education2004 81 (1), 159
- Peer-Reviewed JCE WebWare
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  To celebrate the official initiation of the Journal of Chemical Education Digital Library, this month we add “Kinetica: An Excel Program To Simulate or Analyze Kinetic Data” to our collection of 20 peer-reviewed WebWare programs.
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