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A Simple Device to Demonstrate the Principles of Fluorometry
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Abstract
A simple device to demonstrate the principles of fluorimetry was constructed using low-cost components that are readily obtained. These components were a mini ultraviolet fluorescent lamp, a plastic cell holder, borosilicate glass tubes, and a photoresistor (LDR). The LDR resistance was measured by a multimeter interfaced to a computer for data acquisition and processing. A calibration curve was constructed to relate the fluorescence of quinine bisulfate (QBS) solutions with the LDR resistance. QBS fluorescence quenching by NaCl was studied by the Stern-Volmer approach. The apparatus was designed to permit the direct observation of the following features of the fluorescence phenomenon: (i) the wavelength shift between excitation and emission light, (ii) the increase of emission intensity with increasing fluorophore concentration, and (iii) the inner filter effect for highly concentrated QBS solutions.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Physical ChemistryKeywords (Feature):
Cost-Effective TeacherKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Instrumental MethodsCiting Articles
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This article has been cited by 6 ACS Journal articles (5 most recent appear below).
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- Received: August 03, 2009
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