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Chromatin Isolation and DNA Sequence Analysis in Large Undergraduate Laboratory Sections

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Ann E. Hagerman

Department of Chemistry & Biochemistry, Miami University, Oxford, OH 45056

J. Chem. Educ., 1999, 76 (10), p 1426

DOI: 10.1021/ed076p1426

Publication Date (Web): October 1, 1999

Abstract

A pair of exercises that introduce undergraduate students to basic techniques and concepts of molecular biology and that are appropriate for classes with large enrollments are described. One exercise is a simple laboratory experiment in which chromatin is isolated from chicken liver and is resolved into histone proteins and DNA by ion-exchange chromatography. The other is a series of computer simulations that introduce DNA sequencing, mapping, and sequence analysis to the students. The final step of the simulation is submission of a sequence to a database on the World Wide Web for identification of the protein product of the gene.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Biochemistry

Keywords (Pedagogy):

Computer-Based Learning

Keywords (Subject):

Biotechnology

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