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Reflectance Spectroscopy Using Wine Bottle Glass: An Undergraduate Experiment
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Abstract
Determination of the number of absorbing chromophores in light-scattering media is illustrated in this experiment. Diffuse Reflectance Spectroscopy is used to evaluate this quantity, both for a solid and for a suspension. Results are compared with the absorbance spectrum of the chromophore in a transparent nonscattering matrix.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Laboratory InstructionKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Instrumental MethodsCiting Articles
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This article has been cited by 4 ACS Journal articles (4 most recent appear below).
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Absorption and Scattering Coefficients: A Biophysical-Chemistry Experiment Using Reflectance Spectroscopy
Gabriela B. Cordon and M. Gabriela LagorioJournal of Chemical Education2007 84 (7), 1167Absorption and Scattering Coefficients: A Biophysical-Chemistry Experiment Using Reflectance Spectroscopy
Gabriela B. Cordon and M. Gabriela LagorioJournal of Chemical Education2007 84 (7), 1167The scattering, S, and the absorption, K, coefficients of leaves from different plant species were determined separately as a function of wavelength from reflectance and transmittance data measured for the individual leaves. The equations that link the ...
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María Gabriela LagorioJournal of Chemical Education2004 81 (11), 1607Why Do Marbles Become Paler on Grinding? Reflectance, Spectroscopy, Color, and Particle Size
María Gabriela LagorioJournal of Chemical Education2004 81 (11), 1607Theoretical aspects of light interaction with solid particles are discussed. The dependence of the remission function (from Kubelka–Munk theory) and consequently the modification of the color of a solid with particle size are analyzed in detail. A ...
How Does Light Scattering Affect Luminescence? Fluorescence Spectra and Quantum Yields in the Solid Phase
M. Gabriela Lagorio and Enrique San RománJournal of Chemical Education2002 79 (11), 1362How Does Light Scattering Affect Luminescence? Fluorescence Spectra and Quantum Yields in the Solid Phase
M. Gabriela Lagorio and Enrique San RománJournal of Chemical Education2002 79 (11), 1362Fluorescence quantum yields and luminescence spectra for solid samples are presented and interpreted. Re-absorption of the emitted light is very important in these systems causing a decrease in the observed luminescence quantum yield as well as ...
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- Received: August 03, 2009
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