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An in Vivo 13C NMR Analysis of the Anaerobic Yeast Metabolism of 1-13C-Glucose

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Brent J. Giles , Zenziwe Matsche , Ryan D. Egeland , Ryan A. Reed , Scott S. Morioka and Richard L. Taber

Department of Chemistry, The Colorado College, Colorado Springs, CO 80903

J. Chem. Educ., 1999, 76 (11), p 1564

DOI: 10.1021/ed076p1564

Publication Date (Web): November 1, 1999

Abstract

A biochemistry laboratory experiment that studies the dynamics of the anaerobic yeast metabolism of 1-¹³C-D-glucose via NMR is described. Fleischmann's Active Dry yeast, under anaerobic conditions, produces primarily 2-¹³C-ethanol and some 1-¹³C-glycerol as end products. An experiment is described in which the yeast is subjected to osmotic shock from an increasing sodium chloride concentration. Under these conditions, the yeast increases the ratio of glycerol to ethanol. The experiment can be accomplished in a single laboratory period.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Biochemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Carbohydrates

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This article has been cited by 2 ACS Journal articles (2 most recent appear below).

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