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An Alternative Procedure for the Glucose Oxidase Assay of Glucose as Applied to the Lactase Activity Assay

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T. Corbin Mullis , Jeffery T. Winge and S. Todd Deal

Department of Chemistry, Georgia Southern University, Statesboro, GA 30460-8064

J. Chem. Educ., 1999, 76 (12), p 1711

DOI: 10.1021/ed076p1711

Publication Date (Web): December 1, 1999

Section:

History, Education, and Documentation

Abstract

The glucose oxidase assay of glucose has been modified to eliminate the use of micropipets. The modification involves the use of disposable Pasteur pipets and a specified number of drops of each reagent. This simplified technique gives accurate and reproducible results.

Keywords (Audience):

First-Year Undergraduate / General

Keywords (Domain):

Biochemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Enzymes

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Citing Articles

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This article has been cited by 2 ACS Journal articles (2 most recent appear below).

Application of Datalogger in Biosensing: A Glucose Biosensor
Martin M. F. Choi and Pui Shan Wong
Journal of Chemical Education2002 79 (8), 982
- Application of Datalogger in Biosensing: A Glucose Biosensor
  Martin M. F. Choi and Pui Shan Wong
  Journal of Chemical Education2002 79 (8), 982
  This article describes the use of a datalogger in conjunction with an oxygen sensor to fabricate a glucose biosensor. Glucose oxidase is first covalently immobilized on an eggshell membrane. The membrane is then used to cover the surface of an oxygen ...
  Abstract | Hi-Res PDF | PDF w/ Links
Carbohydrate Analysis: Can We Control the Ripening of Bananas?
S. Todd Deal , Catherine E. Farmer and Paul F. Cerpovicz
Journal of Chemical Education2002 79 (4), 479
- Carbohydrate Analysis: Can We Control the Ripening of Bananas?
  S. Todd Deal , Catherine E. Farmer and Paul F. Cerpovicz
  Journal of Chemical Education2002 79 (4), 479
  We have developed an experiment for nutritional/introductory biochemistry courses that focuses on carbohydrate analysis--specifically, the carbohydrates found in bananas and the change in carbohydrate composition as the banana ripens. Pairs of students ...
  Abstract | Hi-Res PDF | PDF w/ Links