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Pungency Quantitation of Hot Pepper Sauces Using HPLC
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Abstract
The format of the laboratory described here allows students to collectively develop an HPLC method for the quantitation of the two predominant capsaicinoids (capsaicin and dihydrocapsaicin) in hot-pepper products. Each small group of students investigated one of the following aspects of the method: detector wavelength, mobile-phase composition, extraction of capsaicinoids, calibration, and quantitation. The format of the lab forced students to communicate and cooperate to develop this method.
Keywords (Audience):
Second-Year UndergraduateKeywords (Domain):
Analytical ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Instrumental MethodsCiting Articles
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This article has been cited by 8 ACS Journal articles (5 most recent appear below).
Extraction, Purification, and Spectroscopic Characterization of a Mixture of Capsaicinoids
Carl E. Wagner, Thomas M. Cahill, and Pamela A. MarshallJournal of Chemical Education2011 88 (11), 1574-1579Extraction, Purification, and Spectroscopic Characterization of a Mixture of Capsaicinoids
Carl E. Wagner, Thomas M. Cahill, and Pamela A. MarshallJournal of Chemical Education2011 88 (11), 1574-1579This laboratory experiment provides a safe and effective way to instruct undergraduate organic chemistry students about natural-product extraction, purification, and NMR spectroscopic characterization. On the first day, students extract dried habañero ...
HPLC Analysis of α- and β-Acids in Hops
Gary A. Baker , Travis M. Danenhower , Leyna J. Force , Kenneth J. Petersen and Thomas A. BettsJournal of Chemical Education2008 85 (7), 954HPLC Analysis of α- and β-Acids in Hops
Gary A. Baker , Travis M. Danenhower , Leyna J. Force , Kenneth J. Petersen and Thomas A. BettsJournal of Chemical Education2008 85 (7), 954Hops have been used for centuries to impart aroma and bitterness to beer. The cones of the female hop plant contain both essential oils, which include many of the fragrant components of hops, and a collection of compounds known as α- and β-acids that are ...
High-Pressure Liquid Chromatography: Quantitative Analysis of Chinese Herbal Medicine
W. F. Chan and C. W. LinJournal of Chemical Education2007 84 (12), 1982High-Pressure Liquid Chromatography: Quantitative Analysis of Chinese Herbal Medicine
W. F. Chan and C. W. LinJournal of Chemical Education2007 84 (12), 1982An HPLC undergraduate experiment on the analysis of traditional Chinese medicine (TCM) has been developed. Two commonly used herbs (glycyrrhizae radix and cinnamomi ramulus) are studied. Glycyrrhizin, cinnamic acid, and cinnamaldehyde are chosen as ...
Quantitative HPLC Analysis of Rosmarinic Acid in Extracts of Melissa officinalis and Spectrophotometric Measurement of Their Antioxidant Activities
Vera Canelas and Cristina Teixeira da CostaJournal of Chemical Education2007 84 (9), 1502Quantitative HPLC Analysis of Rosmarinic Acid in Extracts of Melissa officinalis and Spectrophotometric Measurement of Their Antioxidant Activities
Vera Canelas and Cristina Teixeira da CostaJournal of Chemical Education2007 84 (9), 1502The students prepare tea samples using different quantities of lemon balm leaves (Melissa officinalis) and measure the rosmarinic acid contents by an HPLC–DAD method. The antioxidant properties of the tea samples are evaluated by a spectrophotometric ...
Determination of Capsaicinoids in Habanero Peppers by Chemometric Analysis of UV Spectral Data
Christopher B. Davis, Carolyn E. Markey, Marianna A. Busch, and Kenneth W. BuschJournal of Agricultural and Food Chemistry2007 55 (15), 5925-5933Determination of Capsaicinoids in Habanero Peppers by Chemometric Analysis of UV Spectral Data
Christopher B. Davis, Carolyn E. Markey, Marianna A. Busch, and Kenneth W. BuschJournal of Agricultural and Food Chemistry2007 55 (15), 5925-5933A novel spectrophotometric method for the determination of capsaicinoids in habanero pepper extracts is described that does not require prior analyte separation. The method uses partial-least-squares (PLS-1) multivariate regression modeling techniques in ...
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- Received: August 03, 2009
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