Internal Standard Calculations in Chromatography

Judy A. Magee and Antony C. Herd
Otago Polytechnic, Department of Applied Science & Technology, Dunedin, NEW ZEALAND
J. Chem. Educ., 1999, 76 (2), p 252
DOI: 10.1021/ed076p252
Publication Date (Web): February 1, 1999

Abstract

This paper outlines a simple approach to internal standard calculations in chromatography. It enables students to cope with an important but troublesome calculation often ignored by undergraduate analytical chemistry texts.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Analytical Chemistry

Keywords (Subject):

Chromatography

Citing Articles

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This article has been cited by 4 ACS Journal articles (4 most recent appear below).

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    Using FTIR-ATR Spectroscopy To Teach the Internal Standard Method

    Michael K. Bellamy
    Journal of Chemical Education2010 87 (12), 1399-1401
    • Using FTIR-ATR Spectroscopy To Teach the Internal Standard Method

      Michael K. Bellamy
      Journal of Chemical Education2010 87 (12), 1399-1401

      The internal standard method is widely applied in quantitative analyses. However, most analytical chemistry textbooks either omit this topic or only provide examples of a single-point internal standardization. An experiment designed to teach students how ...

  • Cover Image

    Measurement of Henry's Law Constants Using Internal Standards. A Quantitative GC Experiment for the Instrumental Analysis or Environmental Chemistry Laboratory

    Chang Ji , Susanne M. Boisvert , Ann-Marie C. Arida and Shannon E. Day
    Journal of Chemical Education2008 85 (7), 969
    • Measurement of Henry's Law Constants Using Internal Standards. A Quantitative GC Experiment for the Instrumental Analysis or Environmental Chemistry Laboratory

      Chang Ji , Susanne M. Boisvert , Ann-Marie C. Arida and Shannon E. Day
      Journal of Chemical Education2008 85 (7), 969

      An internal standard method applicable to undergraduate instrumental analysis or environmental chemistry laboratory has been designed and tested to determine the Henry's law constants for a series of alkyl nitriles. In this method, a mixture of the ...

  • Cover Image

    Quantitative Comparison of Three Standardization Methods Using a One-Way ANOVA for Multiple Mean Comparisons

    Russell D. Barrows
    Journal of Chemical Education2007 84 (5), 839
    • Quantitative Comparison of Three Standardization Methods Using a One-Way ANOVA for Multiple Mean Comparisons

      Russell D. Barrows
      Journal of Chemical Education2007 84 (5), 839

      The use of an external standard calibration curve, standard addition, and internal standard methods for quantitatively determining the concentration of analytes is important to the study of analytical chemistry, especially in the area of instrumental ...

  • Cover Image

    Kinetic Isotope Effect in the Chromic Acid Oxidation of Secondary Alcohols

    Charles E. Harding , Christopher W. Mitchell and Jozsef Devenyi
    Journal of Chemical Education2000 77 (8), 1042
    • Kinetic Isotope Effect in the Chromic Acid Oxidation of Secondary Alcohols

      Charles E. Harding , Christopher W. Mitchell and Jozsef Devenyi
      Journal of Chemical Education2000 77 (8), 1042

      The kinetic isotope effect is an invaluable tool in studying certain organic reaction mechanisms. Two activities involving the technique that are suitable for introductory organic laboratory students are described. A simple competition experiment ...

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History

  • Received: August 03, 2009

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