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Revealing the Backbone Structure of B-DNA from Laser Optical Simulations of Its X-ray Diffraction Diagram
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Abstract
A visible laser and a set of nine specially designed diffraction gratings held on a single slide have been used to build up progressively a classroom optical simulation of the diffraction of X-rays by a B-DNA fiber. The nine optical transforms allow understanding, without recourse to helical diffraction theory, of how the prominent features of the observed diffraction pattern arise from each of the structural parameters of the DNA backbone.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
DemonstrationsKeywords (Subject):
Nucleic acids / DNA / RNACiting Articles
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This article has been cited by 3 ACS Journal articles (3 most recent appear below).
Organizing a High School Chemistry Outreach Event: Celebrating National Chemistry Week and the International Year of Chemistry
Brett R. Bodsgard, Trisha A. Johnson, Roger W. Kugel, Nathan R. Lien, Jaime A. Mueller, and Debra J. MartinJournal of Chemical Education2011 Article ASAPOrganizing a High School Chemistry Outreach Event: Celebrating National Chemistry Week and the International Year of Chemistry
Brett R. Bodsgard, Trisha A. Johnson, Roger W. Kugel, Nathan R. Lien, Jaime A. Mueller, and Debra J. MartinJournal of Chemical Education2011 Article ASAPThis article describes how a small liberal arts college organizes a yearly outreach event for regional high school students to celebrate National Chemistry Week. The event can also serve as a model for outreach during the International Year of Chemistry.
A-DNA and B-DNA: Comparing Their Historical X-ray Fiber Diffraction Images
Amand A. LucasJournal of Chemical Education2008 85 (5), 737A-DNA and B-DNA: Comparing Their Historical X-ray Fiber Diffraction Images
Amand A. LucasJournal of Chemical Education2008 85 (5), 737A-DNA and B-DNA are two secondary molecular conformations (among other allomorphs) that double-stranded DNA drawn into a fiber can assume, depending on the relative water content and other chemical parameters of the fiber. They were the first two forms to ...
X-ray Diffraction and the Discovery of the Structure of DNA. A Tutorial and Historical Account of James Watson and Francis Crick's Use of X-ray Diffraction in Their Discovery of the Double Helix Structure of DNA
David T. CrouseJournal of Chemical Education2007 84 (5), 803X-ray Diffraction and the Discovery of the Structure of DNA. A Tutorial and Historical Account of James Watson and Francis Crick's Use of X-ray Diffraction in Their Discovery of the Double Helix Structure of DNA
David T. CrouseJournal of Chemical Education2007 84 (5), 803A step-by-step method of teaching the X-ray diffraction analysis of DNA using the approach employed by James Watson, Francis Crick, Maurice Wilkins, Rosalind Franklin, and Raymond Gosling at an upper undergraduate and graduate level is described. This ...
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- Received: August 03, 2009
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