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Recombinant Green Fluorescent Protein Isoforms: Exercises To Integrate Molecular Biology, Biochemistry, and Biophysical Chemistry
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Abstract
Using common techniques of molecular biology, students transfect Escherichia coli with cloned DNA (cDNA) plasmids encoding for various isoforms of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria. The different GFP isoforms can then be purified and characterized.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
BiochemistryKeywords (Feature):
Concepts in BiochemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
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- Received: August 03, 2009
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