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Resonance Energy Transfer in Peptides: A Biophysical Laboratory Experiment
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Abstract
Fluorescence excitation and absorption spectra are used to calculate the efficiency of resonance energy transfer and the distance between two chromophores within a small peptide. In this investigation, tyrosine is the donor chromophore and tryptophan is the acceptor chromophore. The experimentally obtained distances are compared to those calculated using a molecular modeling program.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Laboratory InstructionKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Biophysical ChemistryCiting Articles
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This article has been cited by 6 ACS Journal articles (5 most recent appear below).
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- Received: August 03, 2009
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