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Examining the Rate of Renaturation for Genomic DNA Isolated from E. coli: An Integrated Physical Biochemistry Laboratory

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Nayhan Fancy and Andrew F. Mehl

Knox College, Department of Chemistry, Box K-7, Galesburg, IL 61401

J. Chem. Educ., 1999, 76 (5), p 646

DOI: 10.1021/ed076p646

Publication Date (Web): May 1, 1999

Section:

History, Education, and Documentation

Abstract

The second-order rate constant for the renaturation of DNA is determined for E. coli genomic DNA. The procedure involves the initial melting of the double-stranded DNA at high temperature and then an abrupt lowering of the temperature so that renaturation can occur. The absorbance at 260 nm is monitored during the entire procedure.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Biochemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Kinetics

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This article has been cited by 2 ACS Journal articles (2 most recent appear below).

Modern Developments in the Physical Chemistry Laboratory
Samuel A. Abrash
2007 973 (), 115-151
- Modern Developments in the Physical Chemistry Laboratory
  Samuel A. Abrash
  2007 973 (), 115-151
  Developments in the physical chemistry laboratory since the publication of the germinal text by Schwenz and Moore (1) are categorized and reviewed. The categories examined include modern instrumentation, current topics in chemistry, integrated ...
  Abstract | Hi-Res PDF | PDF w/ Links
Thermodynamics of DNA Duplex Formation: A Biophysical Chemistry Laboratory Experiment
Kathleen P. Howard
Journal of Chemical Education2000 77 (11), 1469
- Thermodynamics of DNA Duplex Formation: A Biophysical Chemistry Laboratory Experiment
  Kathleen P. Howard
  Journal of Chemical Education2000 77 (11), 1469
  The goal of this biophysical experiment is to measure thermodynamic properties of a short DNA duplex by melting the ordered native structure (duplex) into the disordered, denatured state (single strands) while monitoring the transition using ultraviolet ...
  Abstract | Hi-Res PDF | PDF w/ Links