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The Preparation of a Fluorescent Dye: A Microscale TLC Experiment

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Daniel S. Calimente , Shannon M. Strand , Shao-Chieh Chang and David E. Lewis

Department of Chemistry & Biochemistry, South Dakota State University, Box 2202, Brookings, SD 57007-0896

J. Chem. Educ., 1999, 76 (1), p 82

DOI: 10.1021/ed076p82

Publication Date (Web): January 1, 1999

Abstract

This paper describes an experiment that allows the instructor to introduce TLC as a technique for analyzing reaction mixtures by monitoring the formation of a fluorescent dye. The experiment also illustrates the effects of eluting solvent on elution order.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Organic Chemistry

Keywords (Feature):

The Microscale Laboratory

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Chromatography

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This article has been cited by 4 ACS Journal articles (4 most recent appear below).

A Fluorogenic Aromatic Nucleophilic Substitution Reaction for Demonstrating Normal-Phase Chromatography and Isolation of Nitrobenzoxadiazole Chromophores
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Journal of Chemical Education2011 88 (1), 98-100
- A Fluorogenic Aromatic Nucleophilic Substitution Reaction for Demonstrating Normal-Phase Chromatography and Isolation of Nitrobenzoxadiazole Chromophores
  Jessie A. Key, Matthew D. Li, and Christopher W. Cairo
  Journal of Chemical Education2011 88 (1), 98-100
  Normal-phase chromatography is an essential technique for monitoring chemical reactions, identifying the presence of specific components, as well as the purification of organic compounds. An experiment to facilitate the instruction and understanding of ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
HRMS Directly From TLC Slides. A Powerful Tool for Rapid Analysis of Organic Mixtures
Natalie J. Smith, Marek A. Domin and Lawrence T. Scott
Organic Letters2008 10 (16), 3493-3496
- HRMS Directly From TLC Slides. A Powerful Tool for Rapid Analysis of Organic Mixtures
  Natalie J. Smith, Marek A. Domin and Lawrence T. Scott
  Organic Letters2008 10 (16), 3493-3496
  High-resolution mass spectra (HRMS) of individual spots on thin-layer chromatography (TLC) slides can now be obtained quickly and easily at atmospheric pressure, with zero sample preparation, using commercially available instrumentation. The method is ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
Analysis and Identification of Acid–Base Indicator Dyes by Thin-Layer Chromatography
Daniel D. Clark
Journal of Chemical Education2007 84 (7), 1186
- Analysis and Identification of Acid–Base Indicator Dyes by Thin-Layer Chromatography
  Daniel D. Clark
  Journal of Chemical Education2007 84 (7), 1186
  Thin-layer chromatography (TLC) is a simple and useful technique for the separation of compounds in a mixture. Organic chemists commonly employ TLC to follow the progression of a reaction, to qualitatively analyze a compound or mixture, to determine ...
  Abstract | Hi-Res PDF | PDF w/ Links
Planar Chromatography
Joseph Sherma
Analytical Chemistry2000 72 (12), 9-26
- Planar Chromatography
  Joseph Sherma
  Analytical Chemistry2000 72 (12), 9-26
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links