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Quantitative Protein Determination from Cellulose Acetate Strip Electrophoresis
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Abstract
A laboratory practical work has been designed for the quantitative determination of serum proteins by means of rapid vertical electrophoresis. A calibration curve was used for the determination of the concentrations of different protein fractions separated by electrophoresis. The results obtained by this method are as accurate as those obtained by traditional ones, with the advantage of greater simplicity and reduced time.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Physical ChemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
ElectrophoresisCiting Articles
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This article has been cited by 1 ACS Journal articles (1 most recent appear below).
Measurement of Iron in Egg Yolk: An Instrumental Analysis Experiment Using Biochemical Principles
Kevin M. Maloney , Emmanuel M. Quiazon and Ramee IndralingamJournal of Chemical Education2008 85 (3), 399Measurement of Iron in Egg Yolk: An Instrumental Analysis Experiment Using Biochemical Principles
Kevin M. Maloney , Emmanuel M. Quiazon and Ramee IndralingamJournal of Chemical Education2008 85 (3), 399The generally accepted method to determine iron content in food is by acid digestion or dry ashing and subsequent flame atomic absorption spectrometry or inductively coupled plasma atomic emission spectrometry. We have developed an experiment that ...
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History
- Received: August 03, 2009
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