Cart
Article
Significant Figures, the Periodic Table, and Mass Spectrometry: The Challenge of Large Biomolecules
Hi-Res PDFPurchase the full-text
- PDF/HTML,
figures/images,
references and tables,
(where available)
Abstract
The number of significant figures required for meaningful mass spectrometric data for biomolecules is considered. Both resolution and calibration issues are addressed. The details of the isotopic clusters expected for acetic acid (C2), bradykinin (C50), ubiquitin (C378), soybean trypsin inhibitor (C892), and glycogen phosphorylase b (C4367) are presented. Although masses are known for isotopes to more than six significant figures, relative abundance data generally contain fewer than four significant figures, which limits the number of significant figures that should be reported for large molecules (> 10,000 Da) and the precision of the periodic table.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
BiochemistryKeywords (Subject):
Mass SpectrometryCiting Articles
Citation data is made available by participants in CrossRef's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search in SciFinder.
This article has been cited by 4 ACS Journal articles (4 most recent appear below).
Incorporating Biological Mass Spectrometry into Undergraduate Teaching Labs, Part 3: De Novo Peptide Sequencing Using Electrospray Tandem Mass Spectrometry
Isaac J. Arnquist and Douglas J. BeussmanJournal of Chemical Education2009 86 (8), 966Incorporating Biological Mass Spectrometry into Undergraduate Teaching Labs, Part 3: De Novo Peptide Sequencing Using Electrospray Tandem Mass Spectrometry
Isaac J. Arnquist and Douglas J. BeussmanJournal of Chemical Education2009 86 (8), 966Tandem mass spectrometry (MS–MS) is an important technique routinely used to provide structural information about analytes of interest. One major application of tandem mass spectrometry is in the field of proteomics, which routinely uses MS–MS experiments ...
Incorporating Biological Mass Spectrometry into Undergraduate Teaching Labs, Part 2: Peptide Identification via Molecular Mass Determination
Isaac J. Arnquist and Douglas J. BeussmanJournal of Chemical Education2009 86 (3), 382Incorporating Biological Mass Spectrometry into Undergraduate Teaching Labs, Part 2: Peptide Identification via Molecular Mass Determination
Isaac J. Arnquist and Douglas J. BeussmanJournal of Chemical Education2009 86 (3), 382Mass spectrometry has become a routine analytical tool in the undergraduate curriculum in the form of GC–MS. While relatively few undergraduate programs have incorporated biological mass spectrometry into their programs, the importance of these techniques,...
Incorporating Biological Mass Spectrometry Into Undergraduate Teaching Labs, Part 1: Identifying Proteins Based on Molecular Mass
Isaac J. Arnquist and Douglas J. BeussmanJournal of Chemical Education2007 84 (12), 1971Incorporating Biological Mass Spectrometry Into Undergraduate Teaching Labs, Part 1: Identifying Proteins Based on Molecular Mass
Isaac J. Arnquist and Douglas J. BeussmanJournal of Chemical Education2007 84 (12), 1971Biological mass spectrometry is an important analytical technique in drug discovery, proteomics, and research at the biology–chemistry interface. Currently, few hands-on opportunities exist for undergraduate students to learn about this technique. With ...
Fundamentals of Biomolecule Analysis by Electrospray Ionization Mass Spectrometry. An Instrumental Analysis Laboratory Experiment
Andrea Weinecke and Victor RyzhovJournal of Chemical Education2005 82 (1), 99Fundamentals of Biomolecule Analysis by Electrospray Ionization Mass Spectrometry. An Instrumental Analysis Laboratory Experiment
Andrea Weinecke and Victor RyzhovJournal of Chemical Education2005 82 (1), 99This article describes an undergraduate instrumental analysis laboratory experiment dealing with fundamentals of electrospray ionization mass spectrometry (ESI–MS). Several important aspects of applications of ESI–MS for analysis of biomolecules are ...
Tools
-
Add to Favorites
-
Download Citation
-
Email a Colleague -
Permalink
Order Reprints
Rights & Permissions
Citation Alerts
History
- Received: August 03, 2009
ACS
Network
Facebook
Tweet This
CiteULike
Newsvine
Digg This
Delicious
