Cart
Article
Acid-Base Titration of (S)-Aspartic Acid: A Circular Dichroism Spectrophotometry Experiment
Hi-Res PDFPurchase the full-text
- PDF/HTML,
figures/images,
references and tables,
(where available)
Abstract
The magnitude of the circular dichroism of (S)-aspartic acid in aqueous solutions at a fixed wavelength varies with the addition of strong base. This laboratory experiment consists of the circular dichroism spectrophotometric acid-base titration of (S)-aspartic acid in dilute aqueous solutions, and the use of the resulting data to determine the ionization constant of the protonated amino group. The work familiarizes students with circular dichroism and illustrates the possibility of performing titrations using a less usual instrumental method of following the course of a reaction. It shows the use of a chiroptical property in the determination of the concentration in solution of an optically active molecule, and exemplifies the use of a spectrophotometric titration in the determination of an ionization constant.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Laboratory InstructionKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Acids / BasesCiting Articles
Citation data is made available by participants in CrossRef's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search in SciFinder.
This article has been cited by 3 ACS Journal articles (3 most recent appear below).
Circular Dichroism Spectroscopy in the Undergraduate Curriculum
Adam R. UrbachJournal of Chemical Education2010 87 (9), 891-893Circular Dichroism Spectroscopy in the Undergraduate Curriculum
Adam R. UrbachJournal of Chemical Education2010 87 (9), 891-893Circular dichroism spectropolarimetry (CD) is a method of optical spectroscopy that seems in most practical ways like UV−visible spectroscopy. The main difference between the two methods is that CD, instead of measuring the absorbance of light as a ...
Preparation, Purification, and Secondary Structure Determination of Bacillus circulans Xylanase. A Modular Laboratory Incorporating Aspects of Molecular Biology, Biochemistry, and Biophysical Chemistry
Sal Russo and Lisa GentileJournal of Chemical Education2006 83 (12), 1850Preparation, Purification, and Secondary Structure Determination of Bacillus circulans Xylanase. A Modular Laboratory Incorporating Aspects of Molecular Biology, Biochemistry, and Biophysical Chemistry
Sal Russo and Lisa GentileJournal of Chemical Education2006 83 (12), 1850An integrated six-week module that allows students to develop biochemistry laboratory techniques in the context of a discovery-oriented project has been developed. This module, suitable for undergraduate third- or fourth-year biochemistry lab, focuses on ...
Spectrophotometric Determination of the Dissociation Constant of an Acid-Base Indicator Using a Mathematical Deconvolution Technique
Krystyn P. Alter , John L. Molloy and Emily D. NiemeyerJournal of Chemical Education2005 82 (11), 1682Spectrophotometric Determination of the Dissociation Constant of an Acid-Base Indicator Using a Mathematical Deconvolution Technique
Krystyn P. Alter , John L. Molloy and Emily D. NiemeyerJournal of Chemical Education2005 82 (11), 1682A laboratory experiment that uses a spectrophotometric method to determine the apparent acid-dissociation constant (Ka') of neutral red, a common acid–base indicator, has been developed. The experiment incorporates a unique data analysis technique using a ...
Full Text HTMLTools
-
Add to Favorites
-
Download Citation
-
Email a Colleague -
Permalink
Order Reprints
Rights & Permissions
Citation Alerts
History
- Received: August 03, 2009
ACS
Network
Facebook
Tweet This
CiteULike
Newsvine
Digg This
Delicious
