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Fluorescence Measurement of Pyrene Wall Adsorption and Pyrene Association with Humic Acids. An Experiment for Physical Chemistry or Instrumental Methods
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Abstract
We describe a two-part fluorescence experiment that measures (i) the surface adsorption of the polycyclic aromatic hydrocarbon (PAH) pyrene to Pyrex, quartz, and LDPE surfaces and (ii) the binding of pyrene to natural humic acids (HA). A model for surface adsorption is presented and used to measure wall adsorption constants for pyrene. Pyrene was found to have the strongest adsorption to LDPE and weaker adsorption to Pyrex and quartz. A fluorescence quenching method is described that allows measurement of the association of pyrene with HA while minimizing competition with pyrene wall adsorption. A model for PAH association with HA is presented and evaluated. Association constants are reported for the association of pyrene with three natural humic acids. The adaptability of the PAH-HA association experiment as a project laboratory is discussed.
Keywords (Domain):
Environmental ChemistryKeywords (Subject):
Fluorescence SpectroscopyCiting Articles
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This article has been cited by 2 ACS Journal articles (2 most recent appear below).
Modern Developments in the Physical Chemistry Laboratory
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Steady-State Fluorescence Anisotropy To Investigate Flavonoids Binding to Proteins
Christine M. Ingersoll and Christen M. StrolloJournal of Chemical Education2007 84 (8), 1313Steady-State Fluorescence Anisotropy To Investigate Flavonoids Binding to Proteins
Christine M. Ingersoll and Christen M. StrolloJournal of Chemical Education2007 84 (8), 1313A laboratory experiment using steady-state fluorescence polarization or anisotropy to determine the binding constant for a flavonoid–protein interaction is described. Using the intrinsic fluorescence of quercetin (a model flavonoid), the fluorescence ...
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- Received: August 03, 2009
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