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Article

Identification of Pharmaceuticals via Computer-Aided Thin Layer Chromatography

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Theodore J. Siek

Analytic Bio-Chemistries Inc., 1680-D Loretta Dr., Feasterville, PA 19053

Anthony J. Macherone Jr.

The Fredric Rieders Family Renaissance Foundation, 3701 Welsh Road, Willow Grove, PA 19090

J. Chem. Educ., 2000, 77 (3), p 366

DOI: 10.1021/ed077p366

Publication Date (Web): March 1, 2000

Abstract

In toxicology laboratories, thin-layer chromatography (TLC) provides a quick and accurate method for qualitative identification of unknown biotoxins that may be present in a fluid or tissue sample. These varied samples are presented to the toxicologist for analysis on a routine basis and also during emergency situations or after a death. The ability of TLC to identify or rule out hundreds of compounds in a single analytical run makes it amenable to emergency toxicology and forensic chemistry. However, the ability to decipher the various reactions and elicit meaningful results from the raw data can take years of experience. It would be useful therefore, for analysts to obtain a firm background in TLC while still in academic training. This analytical laboratory experiment demonstrates the methodology of TLC in its relation to toxicology and forensic chemistry. It is easily adapted for high school seniors or undergraduates and employs experimental techniques associated with TLC and post-lab data analysis with concomitant introduction of concepts. The procedures are designed to introduce the student to the concepts, mechanisms, methodology, and reactions of TLC while building skills in record keeping, data analysis, and deductive reasoning.