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Chemistry and Flatulence: An Introductory Enzyme Experiment

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John R. Hardee , Tina M. Montgomery and Wray H. Jones

Department of Chemistry, Henderson State University, Arkadelphia, AR 71999

J. Chem. Educ., 2000, 77 (4), p 498

DOI: 10.1021/ed077p498

Publication Date (Web): April 1, 2000

Section:

History, Education, and Documentation

Abstract

An inexpensive introductory-level enzyme experiment was developed using raffinose family sugars extracted from green split peas as a substrate and the enzymes alpha-galactosidase and sucrase found in Beano. The reaction studied was the hydrolysis of raffinose family sugars to galactose, glucose, and fructose, and the reaction rate was determined using a retail glucometer to measure the concentration of glucose. Results are given on the effect of substrate concentration, enzyme concentration, temperature, and heavy metals on enzyme activity.

Keywords (Audience):

High School / Introductory Chemistry

Keywords (Domain):

Biochemistry

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Nonmajor Courses

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This article has been cited by 2 ACS Journal articles (2 most recent appear below).

Kinetic Parameters for the Noncatalyzed and Enzyme-Catalyzed Mutarotation of Glucose Using a Blood Glucometer
John R. Hardee, Bryan Delgado, and Wray Jones
Journal of Chemical Education2011 Article ASAP
- Kinetic Parameters for the Noncatalyzed and Enzyme-Catalyzed Mutarotation of Glucose Using a Blood Glucometer
  John R. Hardee, Bryan Delgado, and Wray Jones
  Journal of Chemical Education2011 Article ASAP
  The kinetic parameters for the conversion of α-d-glucose to β-d-glucose were measured using a blood glucometer. The reaction order, rate constant, and Arrhenius activation energy are reported for the noncatalyzed reaction and turnover number and Michaelis ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links
The Garden of Green Organic Chemistry at Hendrix College
Thomas E. Goodwin
2009 1011 (), 37-53
- The Garden of Green Organic Chemistry at Hendrix College
  Thomas E. Goodwin
  2009 1011 (), 37-53
  The Hendrix College organic chemistry laboratories were converted to microscale experiments in 1988 to minimize possible adverse environmental impact, increase lab safety, and decrease generation of waste and costs of waste disposal. As we became aware of ...
  Abstract | Hi-Res PDF | PDF w/ Links