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Separation and Quantification of Preservatives Using Ion Pair HPLC and CZE: An Extended Investigation of Separation Mechanisms

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Mary Boyce and Evadne Spickett

Faculty of Communications, Health and Science, Edith Cowan University, Mount Lawley Campus, Perth, Western Australia 6050

J. Chem. Educ., 2000, 77 (6), p 740

DOI: 10.1021/ed077p740

Publication Date (Web): June 1, 2000

Section:

History, Education, and Documentation

Abstract

This paper describes the separation of preservatives in food and cosmetic creams by reversed-phase high-performance liquid chromatography (HPLC), ion pair HPLC, and capillary zone electrophoresis (CZE). The experiments described here are designed to promote understanding of the separation mechanisms involved and give students an opportunity to compare and evaluate the different techniques.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Analytical Chemistry

Keywords (Feature):

Topics in Chemical Instrumentation

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Chromatography

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This article has been cited by 9 ACS Journal articles (5 most recent appear below).

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Journal of Chemical Education2011 88 (3), 254-256
- Capillary Electrophoresis: Focus on Undergraduate Laboratory Experiments
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  Capillary electrophoresis is a versatile technique well suited to teach concepts that are fundamental to a chemistry degree program. The applicability of capillary electrophoresis to metabolites, DNA, proteins, pharmaceutical compounds, environmental ...
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Student Learning and Evaluation in Analytical Chemistry Using a Problem-Oriented Approach and Portfolio Assessment
Mary C. Boyce , Kuki Singh
Journal of Chemical Education2008 85 (12), 1633
- Student Learning and Evaluation in Analytical Chemistry Using a Problem-Oriented Approach and Portfolio Assessment
  Mary C. Boyce , Kuki Singh
  Journal of Chemical Education2008 85 (12), 1633
  This paper describes a student-focused activity that promotes effective learning in analytical chemistry. Providing an environment where students were responsible for their own learning allowed them to participate at all levels from designing the problem ...
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HPLC Analysis of α- and β-Acids in Hops
Gary A. Baker , Travis M. Danenhower , Leyna J. Force , Kenneth J. Petersen and Thomas A. Betts
Journal of Chemical Education2008 85 (7), 954
- HPLC Analysis of α- and β-Acids in Hops
  Gary A. Baker , Travis M. Danenhower , Leyna J. Force , Kenneth J. Petersen and Thomas A. Betts
  Journal of Chemical Education2008 85 (7), 954
  Hops have been used for centuries to impart aroma and bitterness to beer. The cones of the female hop plant contain both essential oils, which include many of the fragrant components of hops, and a collection of compounds known as α- and β-acids that are ...
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Presentation and Impact of Experimental Techniques in Chemistry
Zbigniew Sojka , Michel Che
Journal of Chemical Education2008 85 (7), 934
- Presentation and Impact of Experimental Techniques in Chemistry
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  Journal of Chemical Education2008 85 (7), 934
  Laboratory and practical courses, where students become familiar with experimental techniques and learn to interpret data and relate them to appropriate theory, play a vital role in chemical education. In the large panoply of currently available ...
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