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Using a Diode Laser for Laser-Induced Fluorescence
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Abstract
The construction and use of a laser fluorimeter from a 635-nm red diode laser and an amplified photodiode detector are described. The low cost and monochromatic nature of diode lasers make them attractive as excitation sources for educational fluorescence experiments. Use of this type of fluorimeter is demonstrated by measuring fluorescence signals for various concentrations of Nile blue A dissolved in methanol; concentrations as low as 1 ppb are easily detected. The use of this instrument for monitoring the decomposition of a dye by an oxidizing agent is demonstrated by measuring the decay of fluorescence as a function of time for a 1 ppm Nile blue A solution after the addition of sodium hypochlorite.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Physical ChemistryKeywords (Feature):
Cost-Effective TeacherKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Dyes / PigmentsCiting Articles
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This article has been cited by 8 ACS Journal articles (5 most recent appear below).
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Benjamin T. Wigton, Balwant S. Chohan, Cole McDonald, Matt Johnson, Doug Schunk, Rod Kreuter, and Dan SykesJournal of Chemical Education2011 Article ASAPA low-cost portable fluorimeter was constructed using a 360 nm LED for excitation and a silicon photodiode for detection. The instrument is simple to operate and has been used to investigate fluorescent whitening agents extracted from various brands of ...
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Laura J. MedhurstJournal of Chemical Education2005 82 (2), 278An environmental chemistry lab has been developed using FTIR spectroscopy to determine and compare the components of automobile exhaust for cold start and warm engine conditions. The results are compared with the EPA certification values for the same ...
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- Received: August 03, 2009
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