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Fluorescence Polarization as a Tool to Pinpoint Vesicle Thermal Phase Transitions
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Abstract
Fluorescence polarization (FP) as a biophysical technique is simplistic in its approach and has potential for the direct determination of ligand-receptor binding and supramolecular associations. Here, students determine the gel-to-liquid transition temperature (Tm) in L-α-dimyristoylphosphatidylcholine (DMPC) model bilayer systems based on steady-state FP of a lipid-tailed fluorescent marker bound therein. First students are exposed to both organized media and emission polarization phenomena and then they apply these fundamentals to readily and accurately (±1-2 °C) determine Tm for a neat DMPC vesicular system.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Laboratory InstructionKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Fluorescence SpectroscopyCiting Articles
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This article has been cited by 4 ACS Journal articles (4 most recent appear below).
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- Received: August 03, 2009
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