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A Modification of a Lactase Experiment by Use of Commercial Test Strips

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Tammy J. Melton

Department of Chemistry, Middle Tennessee State University, Murfreesboro, TN 37132

J. Chem. Educ., 2001, 78 (9), p 1243

DOI: 10.1021/ed078p1243

Publication Date (Web): September 1, 2001

Section:

History, Education, and Documentation

Abstract

A modification of an experiment to monitor the action of the enzyme lactase on the substrate lactose is reported. Previous techniques employed chromatography or spectrophotometry to follow the increasing concentration of glucose as the lactose is hydrolyzed. In this paper the use of commercially available glucose test strips for home diabetic care is described as a new approach for determining the glucose concentrations.

Keywords (Audience):

High School / Introductory Chemistry

Keywords (Domain):

Biochemistry

Keywords (Subject):

Carbohydrates

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

An Alternative Procedure for Carbohydrate Analysis of Bananas: Cheaper and Easier
C. Michele Davis-McGibony , Randall R. Bennett , Arthur D. Bossart II and S. Todd Deal
Journal of Chemical Education2006 83 (10), 1543
- An Alternative Procedure for Carbohydrate Analysis of Bananas: Cheaper and Easier
  C. Michele Davis-McGibony , Randall R. Bennett , Arthur D. Bossart II and S. Todd Deal
  Journal of Chemical Education2006 83 (10), 1543
  A modification of an experiment previously developed for nutritional or introductory biochemistry courses quantifying the carbohydrate content of ripening banana is reported. Earlier investigations employed either chromatography or a colorimetric assay ...
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