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Circular Dichroism of Globular Proteins
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Abstract
This paper describes experiments that teach junior/senior undergraduate students about some of the uses of circular dichroism in characterizing several aspects of globular protein structure. The four experiments are (i) qualitative comparison of CD spectra for globular proteins with different amounts of secondary and tertiary structures, (ii) characterization of secondary and tertiary structure of the A state of cytochrome c, which is believed to resemble the molten globule, (iii) kinetics of lysozyme denaturation, and (iv) helicogenesis of concanavalin A, whereby the predominantly β-sheet protein structure is converted into mostly α-helix. These experiments have the pedagogical advantage of giving students experience with a spectroscopic method that provides more important details about protein structures than is provided by most routine spectroscopic methods.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
BiochemistryKeywords (Feature):
Advanced Chemistry Classroom and LaboratoryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Biophysical ChemistryCiting Articles
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This article has been cited by 5 ACS Journal articles (5 most recent appear below).
A Simple Spreadsheet Program To Simulate and Analyze the Far-UV Circular Dichroism Spectra of Proteins
Luciano A. AbriataJournal of Chemical Education2011 Article ASAPA Simple Spreadsheet Program To Simulate and Analyze the Far-UV Circular Dichroism Spectra of Proteins
Luciano A. AbriataJournal of Chemical Education2011 Article ASAPA simple algorithm was implemented in a spreadsheet program to simulate the circular dichroism spectra of proteins from their secondary structure content and to fit α-helix, β-sheet, and random coil contents from experimental far-UV circular dichroism ...
Circular Dichroism Spectroscopy in the Undergraduate Curriculum
Adam R. UrbachJournal of Chemical Education2010 87 (9), 891-893Circular Dichroism Spectroscopy in the Undergraduate Curriculum
Adam R. UrbachJournal of Chemical Education2010 87 (9), 891-893Circular dichroism spectropolarimetry (CD) is a method of optical spectroscopy that seems in most practical ways like UV−visible spectroscopy. The main difference between the two methods is that CD, instead of measuring the absorbance of light as a ...
Determination of Myoglobin Stability by Circular Dichroism Spectroscopy: Classic and Modern Data Analysis
Andrew F. Mehl , Mary A. Crawford and Lei ZhangJournal of Chemical Education2009 86 (5), 600Determination of Myoglobin Stability by Circular Dichroism Spectroscopy: Classic and Modern Data Analysis
Andrew F. Mehl , Mary A. Crawford and Lei ZhangJournal of Chemical Education2009 86 (5), 600Few laboratory procedures describe the use of circular dichroism (CD) at the undergraduate level. To increase the number of laboratory exercises using CD, a thermal denaturation study of myoglobin using CD is described to assess protein stability. Values ...
Preparation, Purification, and Secondary Structure Determination of Bacillus circulans Xylanase. A Modular Laboratory Incorporating Aspects of Molecular Biology, Biochemistry, and Biophysical Chemistry
Sal Russo and Lisa GentileJournal of Chemical Education2006 83 (12), 1850Preparation, Purification, and Secondary Structure Determination of Bacillus circulans Xylanase. A Modular Laboratory Incorporating Aspects of Molecular Biology, Biochemistry, and Biophysical Chemistry
Sal Russo and Lisa GentileJournal of Chemical Education2006 83 (12), 1850An integrated six-week module that allows students to develop biochemistry laboratory techniques in the context of a discovery-oriented project has been developed. This module, suitable for undergraduate third- or fourth-year biochemistry lab, focuses on ...
The Binding Constant of Estradiol to Bovine Serum Albumin. An Upper-Level Experiment Utilizing Tritium-Labeled Estradiol and Liquid Scintillation Counting
Peihong Liang , Bhavin Adhyaru , Wright L. Pearson and Kathryn R. WilliamsJournal of Chemical Education2006 83 (2), 294The Binding Constant of Estradiol to Bovine Serum Albumin. An Upper-Level Experiment Utilizing Tritium-Labeled Estradiol and Liquid Scintillation Counting
Peihong Liang , Bhavin Adhyaru , Wright L. Pearson and Kathryn R. WilliamsJournal of Chemical Education2006 83 (2), 294Students determine the binding constant of estradiol to bovine serum albumin using 3H-labeled estradiol. The estradiol is equilibrated with excess BSA, and subsequently a small fraction (ca. 1%) of the unbound hormone is extracted using solid-phase ...
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- Received: August 03, 2009
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