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Differential Scanning Calorimetric Study of Bilayer Membrane Phase Transitions. A Biophysical Chemistry Experiment
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Abstract
In a series of experiments designed for a biologically-oriented physical chemistry lab, the gel to liquid-crystalline phase transition of phospholipid bilayers is examined by differential scanning calorimetry. The four interrelated experiments examine the effect of altering lipid bilayer composition on the thermodynamic properties of the phase transition. Bilayer composition is modified by (i) varying the length of the phospholipid acyl chains, (ii) varying the identity of the phosphate headgroup, (iii) adding cholesterol, or (iv) mixing phospholipids of two different length acyl chains. Results relating bilayer structure to phase transitions for all experiments are presented, and the relationship of these experiments to the study of naturally occurring biomembranes is examined.
Keywords (Audience):
Upper-Division UndergraduateKeywords (Domain):
Laboratory InstructionKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Biophysical ChemistryCiting Articles
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This article has been cited by 8 ACS Journal articles (5 most recent appear below).
An Undergraduate Experiment Using Differential Scanning Calorimetry: A Study of the Thermal Properties of a Binary Eutectic Alloy of Tin and Lead
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Ronald P. D’Amelia, Daniel Clark, and William NirodeJournal of Chemical Education2011 Article ASAPAn alloy is an intimate association of two or more metals, with or without a definite composition, which has metallic properties. Heterogeneous alloys, such as tin–lead (Sn/Pb) solders, consist of a mixture of crystalline phases with different ...
Using Compression Isotherms of Phospholipid Monolayers To Explore Critical Phenomena. A Biophysical Chemistry Experiment
Derek E. Gragson , Dan Beaman and Rhiannon PorterJournal of Chemical Education2008 85 (2), 272Using Compression Isotherms of Phospholipid Monolayers To Explore Critical Phenomena. A Biophysical Chemistry Experiment
Derek E. Gragson , Dan Beaman and Rhiannon PorterJournal of Chemical Education2008 85 (2), 272Two experiments are described in which students explore phase transitions and critical phenomena by obtaining compression isotherms of phospholipid monolayers using a Langmuir trough. Through relatively simple analysis of their data students gain a better ...
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Probing the Phase Behavior of Membrane Bilayers Using 31P NMR Spectroscopy
Barry Zee and Kathleen Howard2007 969 (), 234-244Probing the Phase Behavior of Membrane Bilayers Using 31P NMR Spectroscopy
Barry Zee and Kathleen Howard2007 969 (), 234-2443IP nuclear magnetic resonance (NMR) spectroscopy is a powerful method to characterize the phase preferences of aqueous dispersion of phospholipids. Here we show 3lP spectra from both a bilayer-forming and a hexagonal-forming synthetic lipid dispersion ...
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Ronald D'Amelia , Thomas Franks and William F. NirodeJournal of Chemical Education2007 84 (3), 453In first-year general chemistry undergraduate courses, thermodynamics and thermal properties such as melting points and changes in enthalpy (ΔH) and entropy (ΔS) of phase changes are frequently discussed. Typically, classical calorimetric methods of ...
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- Received: August 03, 2009
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