HPLC Determination of Taurine in Sports Drinks

Dale L. Orth
Department of Chemistry, Wisconsin Lutheran College, Milwaukee, WI 53226
J. Chem. Educ., 2001, 78 (6), p 791
DOI: 10.1021/ed078p791
Publication Date (Web): June 1, 2001

Abstract

The amino acid taurine (2-aminoethanesulfonic acid) is present as a nutritional supplement in many sports drinks. An experiment, suitable for a junior-senior level instrumental analysis course, is described to measure the amount of taurine in these sports drinks. A pre-column derivatization with Sanger's reagent, 2,4-dinitrofluorobenzene, is followed by an HPLC separation utilizing a gradient elution, and detection at 360 nm.

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  • Received: August 03, 2009

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