UV-Visible First-Derivative Spectrophotometry Applied to an Analysis of a Vitamin Mixture

F. Aberásturi , A. I. Jiménez , F. Jiménez and J. J. Arias
Departamento de Química Analítica, Nutrición y Bromatología, Universidad de La Laguna, Facultad de Química, E-38071 La Laguna, Tenerife, Spain
J. Chem. Educ., 2001, 78 (6), p 793
DOI: 10.1021/ed078p793
Publication Date (Web): June 1, 2001

Abstract

A simple new experiment that uses UV-vis spectrophotometry to introduce undergraduate chemistry students to multicomponent analysis is presented and a method for the simultaneous determination of three vitamins using derivative spectrophotometry (zero-crossing method) is described. The methodology is simple and easy to apply and allows the determination of folic acid, pyridoxine, and thiamine over the concentration ranges 1.02-14.28, 1.00-16.00, and 6.00-20.00 μg mL-1, respectively. The resulting errors were nearly always less than 5%.

Keywords (Audience):

Second-Year Undergraduate

Keywords (Domain):

Demonstrations

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Undergraduate Research
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  • Received: August 03, 2009

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